DDX19A Recombinant antibody, PBS Only

DDX19A Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230232B12

Cat no : 83106-1-PBS

Synonyms

EC:3.6.4.13, DDX19-like protein, DDX19L, DDX19 DDX19L, ATP-dependent RNA helicase DDX19A

---

Validation Data Gallery

• 

  WB analysis using 83106-1-RR (same clone as 83106-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83106-1-RR (DDX19A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83106-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 83106-1-RR (same clone as 83106-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83106-1-RR (DDX19A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83106-1-PBS in a different storage buffer formulation.

• 

  FC experiment of U2OS using 83106-1-RR (same clone as 83106-1-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug DDX19A Recombinant antibody (83106-1-RR, Clone:230232B12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83106-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83106-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83106-1-RR against Human DDX19A were performed. The affinity constant is below 1 pM.

---

Product Information

83106-1-PBS targets DDX19A in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: DDX19A fusion protein Ag7362
• Full Name: DEAD (Asp-Glu-Ala-As) box polypeptide 19A
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC005162
• Gene Symbol: DDX19A
• Gene ID (NCBI): 55308
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

DDX19A, also known as DDX19L, belongs to the DEAD-box helicase family. DDX19A was identified as a novel cytosolic RNA sensor that could activate the NLRP3 inflammasome during virus infection. In addition, DDX19A was proven to be associated with NADPH oxidase 1 (NOX1)-mediated oxidative stress in tumor necrosis factor (TNF)-α-induced A549 cell (PMID: 33968728). It may represent biomarkers of metastasis and novel therapeutic targets in CSCC patients.