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DDX18 Polyclonal antibody

DDX18 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 28502-1-AP

Synonyms

DDX18, DEAD box protein 18, FLJ33908, MrDb, Myc regulated DEAD box protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DDX18 Polyclonal antibody (28502-1-AP)

    WB analysis using 28502-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28502-1-AP (DDX18 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293T cells using DDX18 Polyclonal antibody (28502-1-AP)

    IP experiment of HEK-293T using 28502-1-AP

    IP result of anti-DDX18 (IP:28502-1-AP, 4ug; Detection:28502-1-AP 1:1500) with HEK-293T cells lysate 1560 ug.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using DDX18 Polyclonal antibody (28502-1-AP)

    IF Staining of HEK-293 using 28502-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using DDX18 antibody (28502-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells, K-562 cells
Positive IP detected inHEK-293T cells
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28502-1-AP targets DDX18 in WB, IF/ICC, IP, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DDX18 fusion protein Ag27457
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 18
Calculated Molecular Weight 75 kDa
Observed Molecular Weight 80-85 kDa
GenBank Accession NumberBC001238
Gene Symbol DDX18
Gene ID (NCBI) 8886
RRIDAB_2881158
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for DDX18 antibody 28502-1-APDownload protocol
IF protocol for DDX18 antibody 28502-1-APDownload protocol
IP protocol for DDX18 antibody 28502-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Wael (Verified Customer) (12-08-2022)

Hek293 cells were fractionate into Nuclear and cytoplasmic fractions. DDX18 shows the expected Nuclear localization, just below the 100KD mark, as expected.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Hek293
DDX18 Antibody Western Blot validation (1:1000 dilution) in Hek293 (Cat no:28502-1-AP)