DDX1 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

DDX1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G10G4

Cat no : 67991-1-PBS

Synonyms

DEAD box protein retinoblastoma, DEAD box protein 1, DBP-RB, DBP RB, ATP-dependent RNA helicase DDX1

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Validation Data Gallery

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  WB analysis using 67991-1-Ig (same clone as 67991-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67991-1-Ig (DDX1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67991-1-PBS in a different storage buffer formulation.

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  WB analysis of 4T1 using 67991-1-Ig (same clone as 67991-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 67991-1-Ig (DDX1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67991-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67991-1-Ig (same clone as 67991-1-PBS)

  WB result of DDX1 antibody (67991-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DDX1 transfected HeLa cells. This data was developed using the same antibody clone with 67991-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67991-1-Ig (same clone as 67991-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67991-1-Ig (DDX1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67991-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67991-1-Ig (same clone as 67991-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67991-1-Ig (DDX1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67991-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse lung using 67991-1-Ig (same clone as 67991-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 67991-1-Ig (DDX1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67991-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse lung using 67991-1-Ig (same clone as 67991-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 67991-1-Ig (DDX1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67991-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skin using 67991-1-Ig (same clone as 67991-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 67991-1-Ig (DDX1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67991-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skin using 67991-1-Ig (same clone as 67991-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 67991-1-Ig (DDX1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67991-1-PBS in a different storage buffer formulation.

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  IHC staining of rat skin using 67991-1-Ig (same clone as 67991-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 67991-1-Ig (DDX1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67991-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat skin using 67991-1-Ig (same clone as 67991-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 67991-1-Ig (DDX1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67991-1-PBS in a different storage buffer formulation.

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Product Information

67991-1-PBS targets DDX1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: DDX1 fusion protein Ag16774
• Full Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 1
• Calculated Molecular Weight: 740 aa, 82 kDa
• Observed Molecular Weight: 82 kDa
• GenBank Accession Number: BC012132
• Gene Symbol: DDX1
• Gene ID (NCBI): 1653
• RRID: AB_2918740
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

DDX1 is a DEAD box protein, which is putative RNA helicases with a characteristic asp-glu-ala-asp (DEAD) box motif. DEAD box proteins involve in translation initiation, splicing, and ribosome and spliceosome assembly by altering RNA secondary structure. As a RNA helicase, DDX1 has a role in RNA clearance at DNA double-strand breaks (DSBs), thereby facilitating the template-guided repair of transcriptionally active regions of the genome.