DDR1 Polyclonal antibody

• Featured Product
• KD/KO Validated

DDR1 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 10536-1-AP

Synonyms

CAK, CD167, Cell adhesion kinase, DDR, DDR1, EDDR1, HGK2, Mammary carcinoma kinase 10, MCK 10, MCK10, NEP, NTRK4, Protein tyrosine kinase 3A, Protein tyrosine kinase RTK 6, PTK3, PTK3A, RTK6, TRK E, TRKE, Tyrosine kinase DDR, Tyrosine protein kinase CAK

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Validation Data Gallery

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  IHC staining of human colon cancer using 10536-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10536-1-AP (DDR1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10536-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10536-1-AP (DDR1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 10536-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10536-1-AP (DDR1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 10536-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10536-1-AP (DDR1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human kidney using 10536-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10536-1-AP (DDR1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 10536-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10536-1-AP (DDR1 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human colon cancer tissue, human kidney tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Product Information

10536-1-AP targets DDR1 in IF, IHC, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DDR1 fusion protein Ag0794
• Full Name: discoidin domain receptor tyrosine kinase 1
• Calculated Molecular Weight: 97 kDa
• GenBank Accession Number: BC008716
• Gene Symbol: DDR1
• Gene ID (NCBI): 780
• RRID: AB_2092081
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,mouse	IF,CoIP	Nat Commun; Endothelial discoidin domain receptor 1 senses flow to modulate YAP activation; Authors - Jiayu Liu ; KD Validated
human	IHC,IF	Bioact Mater; Matrix stiffness exacerbates the proinflammatory responses of vascular smooth muscle cell through the DDR1-DNMT1 mechanotransduction axis.; Authors - Jin Wang ; KD Validated
human	IHC	J Exp Clin Cancer Res; High expression of DDR1 is associated with the poor prognosis in Chinese patients with pancreatic ductal adenocarcinoma.; Authors - Yanmiao Huo
human		Exp Cell Res; Downregulation of CYRI-B promotes migration, invasion and EMT by activating the Rac1-STAT3 pathway in gastric cancer; Authors - Guangyao Liu

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