DDB2 Polyclonal antibody

DDB2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30173-1-AP

Synonyms

DDB p48 subunit, DDB2, DDBB, DNA damage binding protein 2, FLJ34321, UV DDB 2, UV DDB2

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Validation Data Gallery

• 

  WB analysis using 30173-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30173-1-AP (DDB2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 30173-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30173-1-AP (DDB2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 30173-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30173-1-AP (DDB2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A431 cells, PC-12 cells, HCT 116 cells, HeLa cells, HepG2 cells, Jurkat cells, NIH/3T3 cells, mouse kidney tissue, mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30173-1-AP targets DDB2 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DDB2 fusion protein Ag32811
• Full Name: damage-specific DNA binding protein 2, 48kDa
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 48-51 kDa
• GenBank Accession Number: NM_000107
• Gene Symbol: DDB2
• Gene ID (NCBI): 1643
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DDB2, also named as DNA damage-binding protein 2 or UV-damaged DNA-binding protein 2, is a 427 amino acid protein, which contains seven WD repeats and belongs to the WD repeat DDB2/WDR76 family. DDB2 is ubiquitously expressed, with highest levels in corneal endothelium and lowest levels in brain. DDB2 localizes in the nucleus and is required for DNA repair. DDB2 regulates negatively the constitutive expression of the SOD2 gene in breast cancer cells and exerts, at least in part, a control of breast cancer cell growth. DDB2 can play a role as oncogene and may become a promising candidate as a predictive marker in breast cancer.