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DDAH2 Polyclonal antibody

DDAH2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14966-1-AP

Synonyms

DDAH, DDAH 2, DDAH2, DDAHII, Dimethylargininase 2, G6a, NG30, Protein G6a, S phase protein



Tested Applications

Positive WB detected inCOLO 320 cells, mouse colon tissue, rat colon tissue
Positive IP detected inCOLO 320 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14966-1-AP targets DDAH2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DDAH2 fusion protein Ag6831
Full Name dimethylarginine dimethylaminohydrolase 2
Calculated Molecular Weight 29 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC001435
Gene Symbol DDAH2
Gene ID (NCBI) 23564
RRIDAB_2276973
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The DDAH family of proteins is formed by two isoforms, DDAH-1 and DDAH-2, both of which utilize ADMA as their substrate and increase NOS activity. DDAH-2,also named as DDAH, G6A, NG30, is the most prevalent isoform in blood vessels and endothelium and is important for embryonic development. It has also been linked to other diseases, such as hypertension, cerebral hemorrhage, cardiac disease, and diabetes. In cultured human endothelial cells,DDAH-2 had been found only in the cytosol, but in the nuclei of rat vascular smooth muscle cells.(PMID:21898353).

Protocols

Product Specific Protocols
WB protocol for DDAH2 antibody 14966-1-APDownload protocol
IHC protocol for DDAH2 antibody 14966-1-APDownload protocol
IP protocol for DDAH2 antibody 14966-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Oxid Med Cell Longev

Human Tissue Kallikrein 1 Is Downregulated in Elderly Human Prostates and Possesses Potential In Vitro Antioxidative and Antifibrotic Effects in Rodent Prostates.

Authors - Mengyang Zhang

Clin Sci (Lond)

DDAH-2 alleviates contrast medium iopromide-induced acute kidney injury through nitric oxide synthase.

Authors - Heng-Huei Lin
humanWB

FASEB J

Neuropilin-1 maintains dimethylarginine dimethylaminohydrolase 1 expression in endothelial cells, and contributes to protection from angiotensin II-induced hypertension.

Authors - Ying Wang
humanWB

iScience

Dissecting VEGF-induced acute versus chronic vascular hyperpermeability: Essential roles of dimethylarginine dimethylaminohydrolase-1.

Authors - Ying Wang
ratWB,IHC

PLoS One

Involvement of DDAH/ADMA/NOS/cGMP and COX-2/PTGIS/cAMP Pathways in Human Tissue Kallikrein 1 Protecting Erectile Function in Aged Rats.

Authors - Kai Cui
mouse,humanWB

Nat Commun

A multicentric consortium study demonstrates that dimethylarginine dimethylaminohydrolase 2 is not a dimethylarginine dimethylaminohydrolase

Authors - Vinitha N Ragavan
  • KO Validated