DDAH1 Polyclonal antibody

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  WB analysis using 30108-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30108-1-AP (DDAH1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 30108-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30108-1-AP (DDAH1 antibody) at dilution of 1:2000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 30108-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30108-1-AP (DDAH1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 30108-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30108-1-AP (DDAH1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 30108-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 30108-1-AP (DDAH1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse eye using 30108-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using DDAH1 antibody (30108-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

DDAH1 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 30108-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

Dimethylargininase 1, DDAHI, DDAH 1, DDAH

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Tested Applications

• Positive WB detected in: Caco-2 cells, SH-SY5Y cells, mouse brain tissue
• Positive IHC detected in: mouse brain tissue, human stomach cancer tissue, rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse eye tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30108-1-AP targets DDAH1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DDAH1 fusion protein Ag32516
• Full Name: dimethylarginine dimethylaminohydrolase 1
• Calculated Molecular Weight: 31kd
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: NM_012137
• Gene Symbol: DDAH1
• Gene ID (NCBI): 23576
• RRID: AB_2935515
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O94760
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Dimethylarginine dimethylaminohydrolase 1 (DDAH1) is an enzyme that can degrade asymmetric dimethylarginine (ADMA), an endogenous nitric oxide synthase (NOS)inhibitor (PMID:26996393). About 80% of endogenous ADMA is metabolized by DDAH, principally the DDAH1 isoform (PMID:22460174). It was reported that DDAH1 is highly expressed in vascular endothelial cells in hearts (PMID:19917889). The observed molecular weight of DDAH1 is 35-40 kDa in the literature.