DCTD Polyclonal antibody

DCTD Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 16784-1-AP

Synonyms

dCMP deaminase, DCTD, Deoxycytidylate deaminase

---

Validation Data Gallery

• 

  WB analysis of human brain using 16784-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16784-1-AP (DCTD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 16784-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16784-1-AP (DCTD antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 16784-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DCTD antibody (16784-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

---

Tested Applications

• Positive WB detected in: human brain tissue, PC-3 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

16784-1-AP targets DCTD in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DCTD fusion protein Ag10216
• Full Name: dCMP deaminase
• Calculated Molecular Weight: 178 aa, 20 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: BC001286
• Gene Symbol: DCTD
• Gene ID (NCBI): 1635
• RRID: AB_2261528
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DCTD(Deoxycytidylate deaminase) is also named as dCMP deaminase and belongs to the cytidine and deoxycytidylate deaminase family. It catalyzes the deamination of dCMP to dUMP, thus providing the nucleotide substrate for thymidylate synthase. Control of deaminase activity at this juncture in deoxyribonucleotide metabolism is determined by the ratio of dCTP to dTTP in the cell, since the enzyme is allosterically activated by dCTP and inhibited by dTTP(PMID:7685356). It has 2 isoforms produced by alternative splicing and can exsit as a dimer(pMID:8798492).

Publications

Species	Application	Title
human	WB	Int J Cancer; Adult T-cell leukemia-lymphoma acquires resistance to DNA demethylating agents through dysregulation of enzymes involved in pyrimidine metabolism.; Authors - Nao Yoshida-Sakai