DCPS Polyclonal antibody

DCPS Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 28465-1-AP

Synonyms

EC:3.6.1.59, Decapping scavenger enzyme, decapping enzyme, scavenger, DCS-1, DCS1

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Validation Data Gallery

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  WB analysis using 28465-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28465-1-AP (DCPS antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 28465-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DCPS antibody (28465-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, HepG2 cells, mouse testis tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28465-1-AP targets DCPS in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DCPS fusion protein Ag29351
• Full Name: decapping enzyme, scavenger
• Calculated Molecular Weight: 337 aa, 39 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC014532
• Gene Symbol: DCPS
• Gene ID (NCBI): 28960
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DcpS, which is a member of the histidine triad (HIT) superfamily of pyrophosphatases, is the first HIT protein with a defined biological function and identifies the HIT motif as a new mRNA decapping domain. DcpS can encode a 38-40 kDa protein, and has the potential to form a homodimer of 80 kDa(PMID: 12198172).