DCP1B Polyclonal antibody

DCP1B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Reviews (1)

Cat no : 13863-1-AP

Synonyms

mRNA-decapping enzyme 1B, hDcp1b, EC:3.6.1.62, DCP1

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Validation Data Gallery

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  WB analysis using 13863-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13863-1-AP (DCP1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 13863-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13863-1-AP (DCP1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 13863-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13863-1-AP (DCP1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13863-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13863-1-AP (DCP1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat spleen using 13863-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 13863-1-AP (DCP1B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 13863-1-AP

  IP result of anti-DCP1B (IP:13863-1-AP, 4ug; Detection:13863-1-AP 1:500) with HeLa cells lysate 1520ug.

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  IHC staining of human liver cancer using 13863-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13863-1-AP (DCP1B antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver cancer using 13863-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13863-1-AP (DCP1B antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 13863-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DCP1B antibody (13863-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  IF Staining of A549 using 13863-1-AP

  Immunofluorescent analysis of A549 cells using 13863-1-AP (DCP1B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse liver tissue, HeLa cells, human liver tissue, Jurkat cells, rat liver tissue, rat spleen tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

13863-1-AP targets DCP1B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DCP1B fusion protein Ag4845
• Full Name: DCP1 decapping enzyme homolog B (S. cerevisiae)
• Calculated Molecular Weight: 618 aa, 68 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC043437
• Gene Symbol: DCP1B
• Gene ID (NCBI): 196513
• RRID: AB_2090411
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DCP1B belongs to DCP1 family. DCP1B may play a role in the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. It may remove the 7-methyl guanine cap structure mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP.  DCP1B has 2 isoforms with the molecular mass of 56 and 68 kDa. DCP1B is associated with DM-related atherogenesis (PMID:33195466).

Publications

Species	Application	Title
	IF	Int J Mol Med; LSm1 binds to the Dengue virus RNA 3' UTR and is a positive regulator of Dengue virus replication.; Authors - Yangchao Dong

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

George (Verified Customer) (08-19-2019)

Shows up well under concentration used - diffuse in unstressed tissue, forms distinct granular structures following 90 minutes 42oC heat shock. Only tested using 488 secondary antibody but showed up brightly under low power microscopy.

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Cortical Slice Culture