DBR1 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

DBR1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 16019-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of human brain using 16019-1-AP (same clone as 16019-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 16019-1-AP (DBR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16019-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 16019-1-AP (same clone as 16019-1-PBS)

  WB result of DBR1 antibody (16019-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DBR1 transfected HeLa cells. This data was developed using the same antibody clone with 16019-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 16019-1-AP (same clone as 16019-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16019-1-AP (DBR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16019-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 16019-1-AP (same clone as 16019-1-PBS)

  IP result of anti-DBR1 (IP:16019-1-AP, 4ug; Detection:16019-1-AP 1:800) with HeLa cells lysate 1560 ug. This data was developed using the same antibody clone with 16019-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 16019-1-AP (same clone as 16019-1-PBS)

  IP result of anti-DBR1 (IP:16019-1-AP, 4ug; Detection:16019-1-AP 1:500) with mouse brain tissue lysate 1280 ug. This data was developed using the same antibody clone with 16019-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 16019-1-AP (same clone as 16019-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta using 16019-1-AP (DBR1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16019-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 16019-1-AP (same clone as 16019-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta using 16019-1-AP (DBR1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16019-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 16019-1-AP (same clone as 16019-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DBR1 antibody (16019-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 16019-1-PBS in a different storage buffer formulation.

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Product Information

16019-1-PBS targets DBR1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DBR1 fusion protein Ag8830
• Full Name: debranching enzyme homolog 1 (S. cerevisiae)
• Calculated Molecular Weight: 544 aa, 62 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: BC009472
• Gene Symbol: DBR1
• Gene ID (NCBI): 51163
• RRID: AB_2230316
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

DBR1(Lariat debranching enzyme) hydrolyzes 2-prime-to-5-prime branched phosphodiester bonds at the branch point of excised lariat intron RNA and converts them into linear molecules. DBR1 belongs to the lariat debranching enzyme family. Inhibiton of Dbr1 can suppress TDP-43 toxicity in primary neurons, suggesting that Dbr1 could be a potential therapeutic target for ALS and related TDP-43 proteinopathies (PMID: 23104007). This protein has 2 isoforms produced by alternative splicing.