Drebrin Polyclonal antibody

Drebrin Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

20

Cat no : 10260-1-AP

Synonyms

DBN1, drebrin 1, Developmentally-regulated brain protein, DBN 1

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Validation Data Gallery

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  WB analysis using 10260-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10260-1-AP (Drebrin antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10260-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10260-1-AP (DBN1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 10260-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10260-1-AP (Drebrin Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 10260-1-AP

  IP result of anti-Drebrin (IP:10260-1-AP, 4ug; Detection:10260-1-AP 1:2000) with K-562 cells lysate 1200ug.

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  IHC staining of mouse testis using 10260-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10260-1-AP (Drebrin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 10260-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10260-1-AP (Drebrin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 10260-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Drebrin antibody (10260-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 10260-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Drebrin antibody (10260-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 10260-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 10260-1-AP (Drebrin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 10260-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 10260-1-AP (Drebrin antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, K-562 cells, Neuro-2a cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 16 publications below
• IHC: See 4 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Product Information

10260-1-AP targets Drebrin in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Drebrin fusion protein Ag0371
• Full Name: drebrin 1
• Calculated Molecular Weight: 71 kDa
• Observed Molecular Weight: 100-130 kDa
• GenBank Accession Number: BC000283
• Gene Symbol: Drebrin
• Gene ID (NCBI): 1627
• RRID: AB_2230301
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Drebrin (DBN1) is an actin binding and stabilizing protein with roles in endocytosis, formation of dendrite spines in neurons and coordinating cell-cell synapses in immune cells. Preferentially expressed in the brain, it is highly localized in dendritic spines and regulates spine shapes. It has two isoforms that are splice variants, with drebrin A being expressed in neural cells, and drebrin E is present in several other cell types. Recently it has been reported that circulating DBN1 levels correlated with retinal nerve fiber layer defect and may reflect the severity of retinal ganglion cells injury in glaucoma patients.

Publications

Species	Application	Title
mouse	IF	Dev Cell; An integrated approach identifies the molecular underpinnings of murine anterior visceral endoderm migration; Authors - Shifaan Thowfeequ
mouse	IF	PLoS Genet; N-wasp is required for structural integrity of the blood-testis barrier.; Authors - Xiang Xiao
human	IF	Int J Mol Sci; Drebrin Regulates Acetylcholine Receptor Clustering and Organization of Microtubules at the Postsynaptic Machinery; Authors - Paloma Alvarez-Suarez
mouse,rat	WB	Mol Neurobiol; L-3-n-Butylphthalide improves synaptic and dendritic spine plasticity and ameliorates neurite pathology in Alzheimer's disease mouse model and cultured hippocampal neurons.; Authors - Longjian Huang
mouse	WB	Toxicol In Vitro; Bisphenol-A exposure induced neurotoxicity and associated with synapse and cytoskeleton in Neuro-2a cells.; Authors - Zhihong Yin
mouse	WB	Environ Sci Pollut Res Int; Acute lead acetate induces neurotoxicity through decreased synaptic plasticity-related protein expression and disordered dendritic formation in nerve cells.; Authors - Lingli Chen