BRINP1 Monoclonal antibody

BRINP1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2c

Reactivity

human, pig, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2A10E3

Cat no : 60336-1-Ig

Synonyms

BRINP1, DBC1, DBCCR1, deleted in bladder cancer 1, FAM5A, Protein FAM5A

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Validation Data Gallery

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  WB analysis of fetal human brain using 60336-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60336-1-Ig (DBC1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 60336-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 60336-1-Ig (DBC1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 60336-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 60336-1-Ig (DBC1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of MCF-7 using 60336-1-Ig

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed MCF-7 cells using 60336-1-Ig(DBC1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: fetal human brain tissue, mouse brain tissue, pig brain tissue
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

60336-1-Ig targets BRINP1 in WB, IF/ICC, ELISA applications and shows reactivity with human, pig, mouse samples.

• Tested Reactivity: human, pig, mouse
• Host / Isotype: Mouse / IgG2c
• Class: Monoclonal
• Type: Antibody
• Immunogen: BRINP1 fusion protein Ag6588
• Full Name: deleted in bladder cancer 1
• Calculated Molecular Weight: 761 aa, 88 kDa
• Observed Molecular Weight: 88 kDa
• GenBank Accession Number: BC021560
• Gene Symbol: BRINP1
• Gene ID (NCBI): 1620
• RRID: AB_2881445
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BRINP1, also known as DBC1 and FAM5A, inhibits cell proliferation by negative regulation of the G1/S transition. It mediates cell death which is not of the classical apoptotic type and regulates expression of components of the plasminogen pathway.