DAZAP2 Polyclonal antibody

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  WB analysis using 15210-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15210-1-AP (DAZAP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

DAZAP2 Polyclonal Antibody for WB, ELISA

Cat No. 15210-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

PRTB, Proline-rich transcript in brain protein, KIAA0058, Deleted in azoospermia-associated protein 2, DAZ-associated protein 2

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Tested Applications

• Positive WB detected in: A375 cells, C6 cells, J774A.1 cells, RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15210-1-AP targets DAZAP2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DAZAP2 fusion protein Ag7364
• Full Name: DAZ associated protein 2
• Calculated Molecular Weight: 17 kDa
• Observed Molecular Weight: 17 kDa, 22 kDa
• GenBank Accession Number: BC002334
• Gene Symbol: DAZAP2
• Gene ID (NCBI): 9802
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q15038
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DAZAP2, also named KIAA0058, has six isoforms. In unstressed cells, it promotes SIAH1-mediated polyubiquitination and degradation of the serine/threonine-protein kinase HIPK2, probably by acting as a loading factor that potentiates complex formation between HIPK2 and ubiquitin ligase SIAH1. In response to DNA damage, it localizes to the nucleus following phosphorylation by HIPK2 and modulates the expression of a subset of TP53/p53 target genes by binding to TP53 at target gene promoters (PMID: 33591310).