DAO Polyclonal antibody

DAO Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13273-1-AP

Synonyms

D amino acid oxidase, DAAO, DAMOX, DAO, OXDA



Tested Applications

Positive WB detected inmouse kidney tissue, rat cerebellum tissue, A549 cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13273-1-AP targets DAO in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DAO fusion protein Ag3973
Full Name D-amino-acid oxidase
Calculated Molecular Weight 347 aa, 39 kDa
Observed Molecular Weight 39 kDa
GenBank Accession NumberBC029057
Gene Symbol DAO
Gene ID (NCBI) 1610
RRIDAB_2877932
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

D-amino acid oxidase (DAO),with MW of 39 kDa, is a flavoprotein which uses flavin adenine dinucleotide as its prosthetic group. Its substrates include a wide variety of D-amino acids, but it is inactive on the naturally occurring L-amino acids. DAO may play a role in the pathophysiology of schizophrenia and act as a detoxifying agent which removes D-amino acids that accumulate during aging. DAAO exists in solution as a mixture of monomer-dimer(80 kDa) and even higher molecular aggregates in equilibrium(170 kDa), depending on the enzyme concentration and forms(PMID: 11130179/PMID: 9210639).

Protocols

Product Specific Protocols
WB protocol for DAO antibody 13273-1-APDownload protocol
IHC protocol for DAO antibody 13273-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Brain Behav Immun

l-Cysteine suppresses hypoxia-ischemia injury in neonatal mice by reducing glial activation, promoting autophagic flux and mediating synaptic modification via H2S formation.

Authors - Danqing Xin
mouseIF

Am J Physiol Cell Physiol

Functional role of histamine receptors in the renal cortical collecting duct cells.

Authors - Anastasia V Sudarikova
rat

Mol Cell Neurosci

d-Cysteine promotes dendritic development in primary cultured cerebellar Purkinje cells via hydrogen sulfide production.

Authors - Takahiro Seki
human,ratWB,IHC

Am J Physiol Renal Physiol

Renal Histaminergic System and Acute Effects of Histamine Receptor 2 Blockade on Renal Damage in the Dahl Salt-Sensitive Rat

Authors - Denisha R Spires

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Hua (Verified Customer) (03-14-2023)

It does not work in WB.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HepG2, mouse liver, mouse kidney, mouse brain
DAO Antibody Western Blot validation (1:1000 dilution) in HepG2, mouse liver, mouse kidney, mouse brain (Cat no:13273-1-AP)