DAO Polyclonal antibody

DAO Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Reviews (1)

Cat no : 13273-1-AP

Synonyms

D amino acid oxidase, DAAO, DAMOX, DAO, OXDA

---

Validation Data Gallery

• 

  WB analysis of mouse kidney using 13273-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13273-1-AP (DAO antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 13273-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 13273-1-AP (DAO Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat cerebellum using 13273-1-AP

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 13273-1-AP (DAO antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 13273-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13273-1-AP (DAO Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 13273-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13273-1-AP (DAO Antibody) at dilution of 1:200 (under 40x lens).

---

Tested Applications

• Positive WB detected in: mouse kidney tissue, rat cerebellum tissue, A549 cells
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

13273-1-AP targets DAO in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DAO fusion protein Ag3973
• Full Name: D-amino-acid oxidase
• Calculated Molecular Weight: 347 aa, 39 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC029057
• Gene Symbol: DAO
• Gene ID (NCBI): 1610
• RRID: AB_2877932
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

D-amino acid oxidase (DAO),with MW of 39 kDa, is a flavoprotein which uses flavin adenine dinucleotide as its prosthetic group. Its substrates include a wide variety of D-amino acids, but it is inactive on the naturally occurring L-amino acids. DAO may play a role in the pathophysiology of schizophrenia and act as a detoxifying agent which removes D-amino acids that accumulate during aging. DAAO exists in solution as a mixture of monomer-dimer(80 kDa) and even higher molecular aggregates in equilibrium(170 kDa), depending on the enzyme concentration and forms(PMID: 11130179/PMID: 9210639).

Publications

Species	Application	Title
mouse	WB	Brain Behav Immun; l-Cysteine suppresses hypoxia-ischemia injury in neonatal mice by reducing glial activation, promoting autophagic flux and mediating synaptic modification via H2S formation.; Authors - Danqing Xin
mouse	IF	Am J Physiol Cell Physiol; Functional role of histamine receptors in the renal cortical collecting duct cells.; Authors - Anastasia V Sudarikova
rat		Mol Cell Neurosci; d-Cysteine promotes dendritic development in primary cultured cerebellar Purkinje cells via hydrogen sulfide production.; Authors - Takahiro Seki
human,rat	WB,IHC	Am J Physiol Renal Physiol; Renal Histaminergic System and Acute Effects of Histamine Receptor 2 Blockade on Renal Damage in the Dahl Salt-Sensitive Rat; Authors - Denisha R Spires

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Hua (Verified Customer) (03-14-2023)

It does not work in WB.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HepG2, mouse liver, mouse kidney, mouse brain

DAO Antibody Western Blot validation (1:1000 dilution) in HepG2, mouse liver, mouse kidney, mouse brain (Cat no:13273-1-AP)