DAB2IP Polyclonal antibody

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  WB analysis using 30020-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30020-1-AP (DAB2IP antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 30020-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30020-1-AP (DAB2IP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of 4T1 using 30020-1-AP

  IP result of anti-DAB2IP (IP:30020-1-AP, 4ug; Detection:30020-1-AP 1:6000) with 4T1 cells lysate 2800 ug.

• 

  IP experiment of 4T1 using 30020-1-AP

  IP result of anti-DAB2IP (IP:30020-1-AP, 4ug; Detection:30020-1-AP 1:6000) with 4T1 cells lysate 4800 ug.

DAB2IP Polyclonal Antibody for WB, IP, ELISA

Cat No. 30020-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

DAB2 interacting protein, ASK-interacting protein 1, ASK interacting protein 1, AIP-1, AIP1

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Tested Applications

• Positive WB detected in: mouse brain tissue, HepG2 cells, rat brain tissue
• Positive IP detected in: 4T1 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30020-1-AP targets DAB2IP in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DAB2IP fusion protein Ag31340
• Full Name: DAB2 interacting protein
• Calculated Molecular Weight: 1189 aa, 132 kDa
• Observed Molecular Weight: 125-132 kDa
• GenBank Accession Number: BC146762
• Gene Symbol: DAB2IP
• Gene ID (NCBI): 153090
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q5VWQ8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Disabled homolog 2-interacting protein (DAB2IP) is also named as ASK-interacting protein 1 (AIP-1). DAB2IP, is a new member of the Ras GTPase-activating protein family. It interacts directly with DAB2 and has distinct cellular functions such as modulating different signal cascades associated with cell proliferation, survival, apoptosis and metastasis. The aberrant alteration of DAB2IP in cancer is caused by a variety of mechanisms, including the aberrant promoter methylation, histone deacetylation, and others (PMID: 26658103). DAB2IP is reported to bind with Apoptosis signal-regulating kinase 1 (ASK1), and is called ASK1 Interacting Protein (AIP1) (PMID: 12813029). DAB2IP function is that of a tumor suppressor in tumor cell growth, metastasis, and other aspects in cancer progression. In prostate cancer, DAB2IP expression was repressed by promoter methylation and histone modification (PMID: 12446720, PMID: 15817459).