DAB2 Polyclonal antibody

• Featured Product
• KD/KO Validated

DAB2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 10109-2-AP

Synonyms

DOC2, DOC 2, Disabled homolog 2, Differentially-expressed protein 2, Differentially expressed in ovarian carcinoma 2

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Validation Data Gallery

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  WB analysis of HeLa using 10109-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10109-2-AP (DAB2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10109-2-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10109-2-AP (DAB2 antibody) at dilution of 1:16000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10109-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10109-2-AP (DAB2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10109-2-AP

  IP result of anti-DAB2 (IP:10109-2-AP, 4ug; Detection:10109-2-AP 1:2000) with HeLa cells lysate 4000ug.

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  IHC staining of human kidney using 10109-2-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10109-2-AP (DAB2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 10109-2-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10109-2-AP (DAB2 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 10109-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using DAB2 antibody (10109-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  IF Staining of HeLa using 10109-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 10109-2-AP (DAB2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 10109-2-AP

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug DAB2 Polyclonal antibody (10109-2-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

10109-2-AP targets DAB2 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DAB2 fusion protein Ag0162
• Full Name: disabled homolog 2, mitogen-responsive phosphoprotein (Drosophila)
• Calculated Molecular Weight: 82 kDa
• Observed Molecular Weight: 96 kDa
• GenBank Accession Number: BC003064
• Gene Symbol: DAB2
• Gene ID (NCBI): 1601
• RRID: AB_2089700
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DAB2 is a protein of 770 amino acid residues with a predicted molecular weight of 85.5kDa. This gene was initially named DOC2 (for Differentially expressed in Ovarian Cancer) and is distinct from the DOC2A and DOC2B genes (for double C2-like domains, alpha and beta). Human DAB2 has an overall 83% identify with the mouse p96 protein, a putative mitogen-responsive phosphoprotein; homology is strongest in the amino-terminal end of the protein in a region corresponding to the phosphotyrosine interaction domain (PID), and contains multiple SH3 binding motifs. Chromosomal localization by FISH showed that the DAB2 gene is located on 5p13. The expression of DAB2 is down-regulated or absent in all the carcinoma cell lines examined, including prostate and ovarian carcinoma cell lines. The N-terminal domain of DAB2 interacts with Dishevelled-3 (Dvl-3), a signaling mediator of the Wnt pathway. Ectopic expression of DAB2 attenuates canonical Wnt/catenin-mediated signaling, including accumulation of catenin and cyclin D1 induction. DAB2 suppresses both protein kinase C and peptide growth factor-elicited signal pathways via the Ras-mitogen-activated protein kinase pathway. The proline-rich domain of DAB2 also interacts with proteins containing SH3 domain, such as Src and Fgr. The binding of DAB2 to c-Src resultes in the inactivation of c-Src. All data suggest that DAB2 is a potent tumor suppressor in many cancer types.

Publications

Species	Application	Title
Chicken	IP	Proc Natl Acad Sci U S A; FGFR1-mediated protocadherin-15 loading mediates cargo specificity during intraflagellar transport in inner ear hair-cell kinocilia.; Authors - Akira Honda
human	WB	Cancer Lett; MicroRNA-93 promotes cell growth and invasion in nasopharyngeal carcinoma by targeting disabled homolog-2.; Authors - Ya Fei Xu
human	IF	J Cell Physiol; Proper E-cadherin membrane location in colon requires Dab2 and it modifies by inflammation and cancer.; Authors - María D Vazquez-Carretero
human	IHC	Histopathology; Expression and clinical significance of the transforming growth factor-β signalling pathway in endometrial cancer.; Authors - Mhawech-Fauceglia Paulette P
human	WB	J Diabetes Res; Beta-Hydroxybutyric Acid Inhibits Renal Tubular Reabsorption via the AKT/DAB2/Megalin Signalling Pathway; Authors - Minxia Zuo
human	WB,IHC	Dis Markers; Identification and Validation of a Prognostic Signature Based on Methylation Profiles and Methylation-Driven Gene DAB2 as a Prognostic Biomarker in Differentiated Thyroid Carcinoma; Authors - Gaoda Ju