D2HGDH Polyclonal antibody

• Featured Product
• KD/KO Validated

D2HGDH Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 13895-1-AP

Synonyms

EC:1.1.99.39, D-2-hydroxyglutarate dehydrogenase, mitochondrial, D2HGD

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Validation Data Gallery

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  WB analysis of mouse kidney using 13895-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13895-1-AP (D2HGDH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 13895-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13895-1-AP (D2HGDH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 13895-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13895-1-AP (D2HGDH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 13895-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13895-1-AP (D2HGDH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 13895-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13895-1-AP (D2HGDH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 13895-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 13895-1-AP (D2HGDH Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver tissue using 13895-1-AP

  IP result of anti-D2HGDH (13895-1-AP for IP and Detection) with mouse liver tissue lysate.

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  IP experiment of mouse liver using 13895-1-AP

  IP result of anti-D2HGDH (IP:13895-1-AP, 3ug; Detection:13895-1-AP 1:500) with mouse liver tissue lysate 5000ug.

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  IHC staining of human liver cancer using 13895-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13895-1-AP (D2HGDH Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 13895-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13895-1-AP (D2HGDH Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 13895-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 13895-1-AP (D2HGDH antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse kidney tissue, human liver tissue, human heart tissue, rat liver tissue, mouse liver tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 10 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Product Information

13895-1-AP targets D2HGDH in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: D2HGDH fusion protein Ag4857
• Full Name: D-2-hydroxyglutarate dehydrogenase
• Calculated Molecular Weight: 521 aa, 56 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: BC036604
• Gene Symbol: D2HGDH
• Gene ID (NCBI): 728294
• RRID: AB_2089330
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

D2HGDH(D-2-hydroxyglutarate dehydrogenase, mitochondrial) is also named as D2HGD and belongs to the FAD-binding oxidoreductase/transferase type 4 family.It catalyzes the oxidation of D-2-hydroxyglutarate to alpha-ketoglutarate.Defects in D2HGDH are the cause of D-2-hydroxyglutaric aciduria type 1 (D2HGA1).It has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB	Cell Metab; Hypoxia Induces Production of L-2-Hydroxyglutarate.; Authors - Andrew M Intlekofer ; KD Validated
human	WB	Cell Metab; Hypoxia-Mediated Increases in L-2-hydroxyglutarate Coordinate the Metabolic Response to Reductive Stress.; Authors - William M Oldham ; KD Validated
human	WB, IF	Nat Commun; D2HGDH regulates alpha-ketoglutarate levels and dioxygenase function by modulating IDH2.; Authors - An-Ping Lin
human	WB	Cell Chem Biol; MYC Regulation of D2HGDH and L2HGDH Influences the Epigenome and Epitranscriptome.; Authors - ZhiJun Qiu ; KO Validated
human	WB	Leukemia; MYC, mitochondrial metabolism and O-GlcNAcylation converge to modulate the activity and subcellular localization of DNA and RNA demethylases.; Authors - An-Ping Lin
	WB	Oncotarget; D-2-hydroxyglutarate is essential for maintaining oncogenic property of mutant IDH-containing cancer cells but dispensable for cell growth.; Authors - Shenghong Ma