Cytokeratin 6A Monoclonal antibody

Cytokeratin 6A Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

2H6A11

Cat no : 66685-1-Ig

Synonyms

Cytokeratin 6, KRT6A, CK-6A, CK6A, CK-6

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Validation Data Gallery

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  WB analysis of A431 using 66685-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 66685-1-Ig (KRT6A antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 66685-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66685-1-Ig (Cytokeratin 6A antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 66685-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66685-1-Ig (Cytokeratin 6A antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A431 cells
• Positive IHC detected in: human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:20000-1:100000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

66685-1-Ig targets Cytokeratin 6A in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Cytokeratin 6A fusion protein Ag0882
• Full Name: keratin 6A
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC008807
• Gene Symbol: Cytokeratin 6A
• Gene ID (NCBI): 3853
• RRID: AB_2882039
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 6 is a type II keratin. It is used as marker for epidermal hyperproliferation and differentiation. Three are three keratin 6 isoforms: ketatin 6A, 6B and 6C. They share more than 99% identical DNA sequence.

Publications

Species	Application	Title
human	IF,IHC	Aging (Albany NY); Alteration of tumor-associated macrophage subtypes mediated by KRT6A in pancreatic ductal adenocarcinoma.; Authors - Junfeng Zhang
mouse	IF	Cell Tissue Res; Sodium alginate/collagen hydrogel loaded with human umbilical cord mesenchymal stem cells promotes wound healing and skin remodeling.; Authors - Zhenkun Zhang