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CoraLite®555-conjugated Cytokeratin 5 Polyclonal antibody

Cytokeratin 5 Polyclonal Antibody for IF/ICC, IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IF-P

Conjugate

CoraLite®555 Fluorescent Dye

Cat no : CL555-28506

Synonyms

CK 5, CK-5, keratin 5, KRT5, Cytokeratin5

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CoraLite®555-conjugated Cytokeratin 5 Polyclonal a (CL555-28506)

    IF Staining of human tonsillitis using CL555-28506

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®555 Cytokeratin 5 antibody (CL555-28506) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CoraLite®555-conjugated Cytokeratin 5 Polyclonal a (CL555-28506)

    IF Staining of human tonsillitis using CL555-28506

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®555 Cytokeratin 5 antibody (CL555-28506) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CoraLite®555-conjugated Cytokeratin 5 Polyclonal a (CL555-28506)

    IF Staining of A431 using CL555-28506

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using CoraLite®555 Cytokeratin 5 antibody (CL555-28506) at dilution of 1:400.

Tested Applications

Positive IF-P detected inhuman tonsillitis tissue
Positive IF/ICC detected inA431 cells, human tonsillitis tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-28506 targets Cytokeratin 5 in IF/ICC, IF-P applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cytokeratin 5 fusion protein Ag25436
Full Name keratin 5
Calculated Molecular Weight 590 aa, 62 kDa
GenBank Accession NumberBC024292
Gene Symbol Cytokeratin 5
Gene ID (NCBI) 3852
RRIDAB_2919650
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL555 Cytokeratin 5 antibody CL555-28506Download protocol
Standard Protocols
Click here to view our Standard Protocols