Cytokeratin 5 Monoclonal antibody

Cytokeratin 5 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig and More (1)

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

Publications

10

CloneNo.

1A1C5

Reviews (1)

Cat no : 66727-1-Ig

Synonyms

CK 5, CK-5, keratin 5, KRT5, Cytokeratin,Keratin

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Validation Data Gallery

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  WB analysis using 66727-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skin using 66727-1-Ig

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SKOV-3 using 66727-1-Ig

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 66727-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 66727-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skin using 66727-1-Ig

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 66727-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66727-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66727-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:6000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 66727-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:3000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 66727-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:3000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 66727-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:10000 (under 10x lens) showing negative staining. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 66727-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:10000 (under 40x lens) showing negative staining. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66727-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66727-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 66727-1-Ig

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 66727-1-Ig

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66727-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:6000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66727-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:6000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast hyperplasia using 66727-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:6000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast hyperplasia using 66727-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:6000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66727-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:6000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66727-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:6000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast hyperplasia using 66727-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:6000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast hyperplasia using 66727-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:6000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66727-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:6000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 66727-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 66727-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 66727-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 66727-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human oesophagus cancer using 66727-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human oesophagus cancer tissue using Cytokeratin 5 antibody (66727-1-Ig, Clone: 1A1C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human oesophagus cancer using 66727-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human oesophagus cancer tissue using Cytokeratin 5 antibody (66727-1-Ig, Clone: 1A1C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human prostate cancer using 66727-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using Cytokeratin 5 antibody (66727-1-Ig, Clone: 1A1C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human oesophagus cancer using 66727-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human oesophagus cancer tissue using Cytokeratin 5 antibody (66727-1-Ig, Clone: 1A1C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human oesophagus cancer using 66727-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human oesophagus cancer tissue using Cytokeratin 5 antibody (66727-1-Ig, Clone: 1A1C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human prostate cancer using 66727-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using Cytokeratin 5 antibody (66727-1-Ig, Clone: 1A1C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of A431 using 66727-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cytokeratin 5 antibody (66727-1-Ig, Clone: 1A1C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HaCaT cells, SKOV-3 cells, mouse skin tissue, MCF-7 cells, A549 cells, A-253 cells, A431 cells, human saliva, pig skin tissue
• Positive IHC detected in: human tonsillitis tissue, human breast cancer tissue, human breast hyperplasia tissue, human lung cancer tissue, human oesophagus cancer tissue, human cervical cancer tissue, human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human oesophagus cancer tissue, human prostate cancer tissue
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:20000-1:100000
• Immunohistochemistry (IHC): IHC : 1:5000-1:20000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 3 publications below
• IF: See 4 publications below

Product Information

66727-1-Ig targets Cytokeratin 5 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, pig samples.

• Tested Reactivity: human, mouse, pig
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Cytokeratin 5 fusion protein Ag24184
• Full Name: keratin 5
• Calculated Molecular Weight: 590 aa, 62 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC024292
• Gene Symbol: Cytokeratin 5
• Gene ID (NCBI): 3852
• RRID: AB_2882077
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18). Keratin 5, one 58-kD type II keratin, is coexpressed with a 50-kD keratin 14 in stratified squamous epithelia. Keratin 5, one 58-kD type II keratin, is coexpressed with a 50-kD tyK14 in stratified squamous epithelia.

Publications

Species	Application	Title
rat	IF	Cell Mol Biol Lett; The role of NPY2R/NFATc1/DYRK1A regulatory axis in sebaceous glands for sebum synthesis; Authors - Tao Yang
mouse	IHC	Front Genet; The Unique Genetic and Histological Characteristics of DMBA-Induced Mammary Tumors in an Organoid-Based Carcinogenesis Model.; Authors - Mie Naruse
human	WB	PLoS One; Construction of ceRNA network to identify the lncRNA and mRNA related to non-small cell lung cancer.; Authors - Kui Xiao
human	WB	Cell Death Dis; SOX1 promotes differentiation of nasopharyngeal carcinoma cells by activating retinoid metabolic pathway.; Authors - Xin-Xing Lei
human	WB	Cell Death Discov; TRIM29 hypermethylation drives esophageal cancer progression via suppression of ZNF750; Authors - Qiyi Yi
mouse	IHC	Immunobiology; High expression of NF-κB inducing kinase in the bulge region of hair follicle induces tumor; Authors - Weisong Duan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Emma (Verified Customer) (11-29-2021)

Used by IF on FFPE prostate tissue. Tris-EFTA antigen retrieval.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Prostate

Cytokeratin 5 Antibody Immunofluorescence validation (1:100 dilution) in Prostate (Cat no:66727-1-Ig)