CoraLite®594-conjugated Cytokeratin 20 Monoclonal antibody

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  IF Staining of HT-29 using CL594-60183

  Immunofluorescent analysis of (-20°C Methanol) fixed HT-29 cells using CoraLite®594 Cytokeratin 20 antibody (CL594-60183, Clone: 4D10A4 ) at dilution of 1:200.

Cytokeratin 20 Monoclonal Antibody for IF/ICC

Cat No. CL594-60183

Clone No.4D10A4

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF/ICC

Synonyms

Cell and organelle markers, CK 20, CK20, Cytokeratin 20, Cytoskeleton Marker, K20, keratin 20, KRT20, KRT21, Protein IT

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Tested Applications

• Positive IF/ICC detected in: HT-29 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-60183 targets Cytokeratin 20 in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11336

  Product name: Recombinant human KRT20 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 74-424 aa of BC031559

  Sequence: MQNLNDRLASYLEKVRTLEQSNSKLEVQIKQWYETNAPRAGRDYSAYYRQIEELRSQIKDAQLQNARCVLQIDNAKLAAEDFRLKYETERGIRLTVEADLQGLNKVFDDLTLHKTDLEIQIEELNKDLALLKKEHQEEVDGLHKHLGNTVNVEVDAAPGLNLGVIMNEMRQKYEVMAQKNLQEAKEQFERQTAVLQQQVTVNTEELKGTEVQLTELRRTSQSLEIELQSHLSMKESLEHTLEETKARYSSQLANLQSLLSSLEAQLMQIRSNMERQNNEYHILLDIKTRLEQEIATYRRLLEGEDVKTTEYQLSTLEERDIKKTRKIKTVVQEVVDGKVVSSEVKEVEENI

• Full Name: keratin 20
• Calculated Molecular Weight: 424 aa, 48 kDa
• GenBank Accession Number: BC031559
• Gene Symbol: Cytokeratin 20
• Gene ID (NCBI): 54474
• RRID: AB_2919900
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P35900
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18).Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa.