Cytokeratin 19 Polyclonal antibody

Cytokeratin 19 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 29855-1-AP

Synonyms

CK19, CK 19, KRT19, K19, Cytokeratin-19

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Validation Data Gallery

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  WB analysis using 29855-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 29855-1-AP (Cytokeratin 19 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 29855-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 29855-1-AP (Cytokeratin 19 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 29855-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 29855-1-AP (Cytokeratin 19 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 29855-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 29855-1-AP (Cytokeratin 19 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human normal colon using 29855-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 29855-1-AP (Cytokeratin 19 antibody) at dilution of 1:8000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 29855-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29855-1-AP (Cytokeratin 19 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 29855-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29855-1-AP (Cytokeratin 19 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 29855-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 29855-1-AP (Cytokeratin 19 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 29855-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 29855-1-AP (Cytokeratin 19 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 29855-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cytokeratin 19 antibody (29855-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A549 using 29855-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using Cytokeratin 19 antibody (29855-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 29855-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Cytokeratin 19 antibody (29855-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: BxPC-3 cells, HT-29 cells, HepG2 cells, MCF-7 cells, T-47D cells
• Positive IHC detected in: human breast cancer tissue, human colon tissue, human thyroid cancer tissue, human normal colon; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, HeLa cells, A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29855-1-AP targets Cytokeratin 19 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cytokeratin 19 fusion protein Ag31469
• Full Name: keratin 19
• Calculated Molecular Weight: 44KD
• Observed Molecular Weight: 40-44 kDa
• GenBank Accession Number: NM_002276
• Gene Symbol: Cytokeratin 19
• Gene ID (NCBI): 3880
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 19 is a type I cytokeratin. It is a biochemical marker of skin stem cells in vivo and in vitro.