Cytokeratin 18 Recombinant antibody, PBS Only

Cytokeratin 18 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4F2

Cat no : 82817-5-PBS

Synonyms

KRT18, Cytokeratin,Keratin, CK-18, CK18, CK 18

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Validation Data Gallery

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  WB analysis using 82817-5-RR (same clone as 82817-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82817-5-RR (KRT18 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82817-5-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 82817-5-RR (same clone as 82817-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 82817-5-RR (Cytokeratin 18 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82817-5-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 82817-5-RR (same clone as 82817-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 82817-5-RR (Cytokeratin 18 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82817-5-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 82817-5-RR (same clone as 82817-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 82817-5-RR (Cytokeratin 18 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82817-5-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 82817-5-RR (same clone as 82817-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 82817-5-RR (Cytokeratin 18 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82817-5-PBS in a different storage buffer formulation.

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  IHC staining of human renal cell carcinoma using 82817-5-RR (same clone as 82817-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 82817-5-RR (Cytokeratin 18 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82817-5-PBS in a different storage buffer formulation.

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  IHC staining of human renal cell carcinoma using 82817-5-RR (same clone as 82817-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 82817-5-RR (Cytokeratin 18 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82817-5-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 82817-5-RR (same clone as 82817-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 82817-5-RR (Cytokeratin 18 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82817-5-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 82817-5-RR (same clone as 82817-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 82817-5-RR (Cytokeratin 18 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82817-5-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 82817-5-RR (same clone as 82817-5-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Cytokeratin 18 antibody (82817-5-RR, Clone: 4F2 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 82817-5-PBS in a different storage buffer formulation.

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Product Information

82817-5-PBS targets Cytokeratin 18 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Cytokeratin 18 fusion protein Ag1260
• Full Name: keratin 18
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC000180
• Gene Symbol: Cytokeratin 18
• Gene ID (NCBI): 3875
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Cytokeratin 18, also known as CK18, is a type I intermediate filament protein which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. CK18 and its coexpressed complementary type II keratin partner, CK8, are persistently expressed in a variety of adult epithelial organs, such as the liver, lung, kidney, pancreas, gastrointestinal tract, and mammary gland, and are also expressed by cancers that arise from these tissues. In the absence of CK8, the CK18 protein is degraded and keratin intermediate filaments are not formed.