Cytokeratin 15 Monoclonal antibody, PBS Only (Capture)

Cytokeratin 15 Monoclonal Antibody for WB, IF, FC, IHC, Sandwich ELISA, Indirect ELISA, Cytometric bead array

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IF, FC, IHC, Sandwich ELISA, Indirect ELISA, Cytometric bead array

Conjugate

Unconjugated

CloneNo.

6B4F8

Cat no : 60247-1-PBS

Synonyms

CK 15, CK15, Cytokeratin 15, K15, K1CO, keratin 15, KRT15, KRTB

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Validation Data Gallery

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  WB analysis of A431 using 60247-1-Ig (same clone as 60247-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 60247-1-Ig (Cytokeratin 15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60247-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP50002-1

  Sandwich ELISA standard curve ofMP50002-1, KRT15 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60247-1-PBS. Detection antibody: HRP-conjugated 60247-2-PBS. Standard: Ag0185. Range: 0.313-20 ng/mL.

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  IHC staining of human oesophagus cancer using 60247-1-Ig (same clone as 60247-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60247-1-Ig (Cytokeratin 15 antibody at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 60247-1-PBS in a different storage buffer formulation.

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  IHC staining of human oesophagus cancer using 60247-1-Ig (same clone as 60247-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60247-1-Ig (Cytokeratin 15 antibody at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 60247-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 60247-1-Ig (same clone as 60247-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 60247-1-Ig (Cytokeratin 15 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60247-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 60247-1-Ig (same clone as 60247-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 60247-1-Ig (Cytokeratin 15 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60247-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 60247-1-Ig (same clone as 60247-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Cytokeratin 15 antibody (60247-1-Ig, Clone: 6B4F8 ) at dilution of 1:25 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60247-1-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 60247-1-Ig (same clone as 60247-1-PBS)

  1X10^6 A431 cells were intracellularly stained with 0.2 ug Anti-Human Cytokeratin 15 (60247-1-Ig, Clone:6B4F8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH. This data was developed using the same antibody clone with 60247-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50002-1

  Cytometric bead array standard curve ofMP50002-1, KRT15 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60247-1-PBS. Detection antibody: 60247-2-PBS. Standard: Ag0185. Range: 1.563-100 ng/mL.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60247-1-PBS targets Cytokeratin 15 as part of a matched antibody pair:

MP50002-1: 60247-1-PBS capture and 60247-2-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Cytokeratin 15 fusion protein Ag0185
• Full Name: keratin 15
• Calculated Molecular Weight: 49 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC002641
• Gene Symbol: KRT15
• Gene ID (NCBI): 3866
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: 100% PBS pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18). Keratin 15 is a type I cytokeratin. It is found in some progenitor basal cells within complex epithelia.