Cystatin C Recombinant antibody

Cystatin C Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240202A2

Cat no : 83422-1-RR

Synonyms

Cst3, CysC, 240202A2

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Validation Data Gallery

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  WB analysis using 83422-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83422-1-RR (cystatin c antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 83422-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 83422-1-RR (cystatin c antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 83422-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 83422-1-RR (cystatin c antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 83422-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83422-1-RR against Mouse Cystatin C were performed. The affinity constant is 20.3 nM.

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Tested Applications

• Positive WB detected in: RAW 264.7 cells, mouse brain tissue
• Positive IHC detected in: mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83422-1-RR is Cystatin C Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: cystatin C
• Calculated Molecular Weight: 15KD
• Observed Molecular Weight: 13-16 kDa
• GenBank Accession Number: NM_009976.4
• Gene Symbol: Cystatin C
• Gene ID (NCBI): 13010
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cystatin C is a 13-kDa inhibitor of cysteine proteinases which is secreted by all cell types and is completely cleared from the organism through glomerular filtration, shown to be an early and sensitive biomarker of renal dysfunction. It is also used as an emerging biomarker in cardiovascular disease. Cystatin C is involved in a variety of inflammatory reactions. The concentration of serum cystatin C has also been shown to be unaltered in certain inflammatory conditions or other disorders of metabolism. The plasma level of serum cystatin C can be expressed as its level of generation from cells and diet and its subsequent elimination through the gut, liver, and kidney.