Cystatin C Recombinant antibody, PBS Only

Cystatin C Uni-rAb^TM Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1I20

Cat no : 82441-1-PBS

Synonyms

CST3, 1I20, Gamma-trace, Cystatin-C, Cystatin-3

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Validation Data Gallery

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  WB analysis using 82441-1-RR (same clone as 82441-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82441-1-RR (Cystatin C antibody) at dilution of 1:3300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82441-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 82441-1-RR (same clone as 82441-1-PBS)

  WB result of Cystatin C antibody (82441-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cystatin C transfected HepG2 cells. This data was developed using the same antibody clone with 82441-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 82441-1-RR (same clone as 82441-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 82441-1-RR (Cystatin C antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82441-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 82441-1-RR (same clone as 82441-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 82441-1-RR (Cystatin C antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82441-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82441-1-PBS

  Biolayer interferometry (BLl) kinetic assays of 82441-1-RR against Human Cystatin C were performed. The affinity constant is below 1 pM.

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Product Information

82441-1-PBS targets Cystatin C in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Cystatin C fusion protein Ag2890
• Full Name: cystatin C
• Calculated Molecular Weight: 146 aa, 16 kDa
• Observed Molecular Weight: 13 kDa
• GenBank Accession Number: BC013083
• Gene Symbol: Cystatin C
• Gene ID (NCBI): 1471
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Cystatin C is a 13-kDa inhibitor of cysteine proteinases which is secreted by all cell types and is completely cleared from the organism through glomerular filtration, shown to be an early and sensitive biomarker of renal dysfunction. It is also used as an emerging biomarker in cardiovascular disease. Cystatin C is involved in a variety of inflammatory reactions. The concentration of serum cystatin C has also been shown to be unaltered in certain inflammatory conditions or other disorders of metabolism. The plasma level of serum cystatin C can be expressed as its level of generation from cells and diet and its subsequent elimination through the gut, liver, and kidneys.