Cyclophilin A Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

Cyclophilin A Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 10720-1-PBS

Synonyms

PPIA, Peptidyl-prolyl cis-trans isomerase A, EC:5.2.1.8, CYPH, CYPA

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Validation Data Gallery

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  WB analysis using 10720-1-AP (same clone as 10720-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10720-1-AP (Cyclophilin A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10720-1-PBS in a different storage buffer formulation.

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  WB analysis using 10720-1-AP (same clone as 10720-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10720-1-AP (Cyclophilin A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10720-1-PBS in a different storage buffer formulation.

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  WB analysis of C6 using 10720-1-AP (same clone as 10720-1-PBS)

  C6 cells were subjected to SDS PAGE followed by western blot with 10720-1-AP (Cyclophilin A antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10720-1-PBS in a different storage buffer formulation.

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  WB analysis of Raji using 10720-1-AP (same clone as 10720-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 10720-1-AP (Cyclophilin A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10720-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 10720-1-AP (same clone as 10720-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10720-1-AP (Cyclophilin A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10720-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 10720-1-AP (same clone as 10720-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10720-1-AP (Cyclophilin A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10720-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 10720-1-AP (same clone as 10720-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10720-1-AP (Cyclophilin A antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10720-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 10720-1-AP (same clone as 10720-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10720-1-AP (Cyclophilin A antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 10720-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 10720-1-AP (same clone as 10720-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 10720-1-AP (Cyclophilin A antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 10720-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 10720-1-AP (same clone as 10720-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta using 10720-1-AP (Cyclophilin A antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10720-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 10720-1-AP (same clone as 10720-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta using 10720-1-AP (Cyclophilin A antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 10720-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 10720-1-AP (same clone as 10720-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 10720-1-AP (Cyclophilin A antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10720-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 10720-1-AP (same clone as 10720-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 10720-1-AP (Cyclophilin A antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10720-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 10720-1-AP (same clone as 10720-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 10720-1-AP (Cyclophilin A antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 10720-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 10720-1-AP (same clone as 10720-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Cyclophilin A antibody (10720-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10720-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 10720-1-AP (same clone as 10720-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Cyclophilin A Polyclonal antibody (10720-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 10720-1-PBS in a different storage buffer formulation.

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Product Information

10720-1-PBS targets Cyclophilin A in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cyclophilin A fusion protein Ag1120
• Full Name: peptidylprolyl isomerase A (cyclophilin A)
• Calculated Molecular Weight: 18 kDa
• Observed Molecular Weight: 18 kDa
• GenBank Accession Number: BC005320
• Gene Symbol: PPIA
• Gene ID (NCBI): 5478
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Cyclophilin A, also named as PPIA and Rotamase A, belongs to the cyclophilin-type PPIase family and PPIase A subfamily. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIA forms a trimolecular complex with cyclophilin and calcineurins to inhibit calcineurin phosphatase activity. PPIA is incorporated into the virion of the type 1 human immunodeficiency virus (HIV-1) via a direct interaction with the capsid domain of the viral Gag polyprotein and is crucial for efficient viral replication.