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Cyclophilin A Polyclonal antibody

Name: Cyclophilin A Polyclonal antibody
Brand: Proteintech
SKU: 10720-1-AP

Cyclophilin A Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10720-1-AP

Synonyms

PPIA, Peptidyl-prolyl cis-trans isomerase A, EC:5.2.1.8, CYPH, CYPA

Validation Data Gallery

Filter:

AllWBIHCIF/ICCFC (Intra)

WB analysis using 10720-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10720-1-AP (Cyclophilin A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis using 10720-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10720-1-AP (Cyclophilin A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of C6 using 10720-1-AP
C6 cells were subjected to SDS PAGE followed by western blot with 10720-1-AP (Cyclophilin A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of Raji using 10720-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 10720-1-AP (Cyclophilin A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human colon cancer using 10720-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10720-1-AP (Cyclophilin A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 10720-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10720-1-AP (Cyclophilin A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 10720-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 10720-1-AP (Cyclophilin A antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human colon cancer using 10720-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 10720-1-AP (Cyclophilin A antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human placenta using 10720-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 10720-1-AP (Cyclophilin A antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human placenta using 10720-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 10720-1-AP (Cyclophilin A antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human testis using 10720-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 10720-1-AP (Cyclophilin A antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human testis using 10720-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 10720-1-AP (Cyclophilin A antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human tonsillitis using 10720-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis using 10720-1-AP (Cyclophilin A antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human tonsillitis using 10720-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis using 10720-1-AP (Cyclophilin A antibody) at dilution of 1:100 (under 40x lens).
IF Staining of HepG2 using 10720-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Cyclophilin A antibody (10720-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HeLa using 10720-1-AP
1x10^6 HeLa cells were intracellularly stained with 0.25 ug Cyclophilin A Polyclonal antibody (10720-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 10720-1-AP

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Tested Applications

Positive WB detected in	HeLa cells, C6 cells, Raji cells, HepG2 cells, K-562 cells, NIH/3T3 cells
Positive IHC detected in	human colon cancer tissue, human placenta tissue, human tonsillitis tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 22 publications below
IHC	See 10 publications below
IF	See 6 publications below

Product Information

10720-1-AP targets Cyclophilin A in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, monkey
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Cyclophilin A fusion protein Ag1120
Full Name	peptidylprolyl isomerase A (cyclophilin A)
Calculated Molecular Weight	18 kDa
Observed Molecular Weight	18 kDa
GenBank Accession Number	BC005320
Gene Symbol	PPIA
Gene ID (NCBI)	5478
RRID	AB_2237516
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cyclophilin A, also named as PPIA and Rotamase A, belongs to the cyclophilin-type PPIase family and PPIase A subfamily. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIA forms a trimolecular complex with cyclophilin and calcineurins to inhibit calcineurin phosphatase activity. PPIA is incorporated into the virion of the type 1 human immunodeficiency virus (HIV-1) via a direct interaction with the capsid domain of the viral Gag polyprotein and is crucial for efficient viral replication.

Protocols

Product Specific Protocols
WB protocol for Cyclophilin A antibody 10720-1-AP	Download protocol
IHC protocol for Cyclophilin A antibody 10720-1-AP	Download protocol
IF protocol for Cyclophilin A antibody 10720-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Res Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2. Authors - Shaojun Zhang View Article
mouse	WB	Sci Adv CGG repeat RNA G-quadruplexes interact with FMRpolyG to cause neuronal dysfunction in fragile X-related tremor/ataxia syndrome. Authors - Sefan Asamitsu View Article
mouse	WB	Brain Defective cyclophilin A induces TDP-43 proteinopathy: implications for amyotrophic lateral sclerosis and frontotemporal dementia Authors - Laura Pasetto KO Validated View Article
human	WB	Mol Neurodegener Decoding distinctive features of plasma extracellular vesicles in amyotrophic lateral sclerosis. Authors - Laura Pasetto View Article
human,mouse	WB,IHC	Inflamm Regen Testosterone upregulates glial cell line-derived neurotrophic factor (GDNF) and promotes neuroinflammation to enhance glioma cell survival and proliferation Authors - Kouminin Kanwore View Article
human	WB,IHC,IF	Virol Sin Cyclophilin A binds to AKT1 and facilitates the tumorigenicity of Epstein-Barr virus by mediating the activation of AKT/mTOR/NF-κB positive feedback loop Authors - Shuyu Xin KO Validated View Article

Cyclophilin A Polyclonal antibody

Cyclophilin A Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Validation Data Gallery

WB analysis using 10720-1-AP

WB analysis using 10720-1-AP

WB analysis of C6 using 10720-1-AP

WB analysis of Raji using 10720-1-AP

IHC staining of human colon cancer using 10720-1-AP

IHC staining of human colon cancer using 10720-1-AP

IHC staining of human colon cancer using 10720-1-AP

IHC staining of human colon cancer using 10720-1-AP

IHC staining of human placenta using 10720-1-AP

IHC staining of human placenta using 10720-1-AP

IHC staining of human testis using 10720-1-AP

IHC staining of human testis using 10720-1-AP

IHC staining of human tonsillitis using 10720-1-AP

IHC staining of human tonsillitis using 10720-1-AP

IF Staining of HepG2 using 10720-1-AP

FC experiment of HeLa using 10720-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2.

CGG repeat RNA G-quadruplexes interact with FMRpolyG to cause neuronal dysfunction in fragile X-related tremor/ataxia syndrome.

Defective cyclophilin A induces TDP-43 proteinopathy: implications for amyotrophic lateral sclerosis and frontotemporal dementia

Decoding distinctive features of plasma extracellular vesicles in amyotrophic lateral sclerosis.

Testosterone upregulates glial cell line-derived neurotrophic factor (GDNF) and promotes neuroinflammation to enhance glioma cell survival and proliferation

Cyclophilin A binds to AKT1 and facilitates the tumorigenicity of Epstein-Barr virus by mediating the activation of AKT/mTOR/NF-κB positive feedback loop