Cyclin G Polyclonal antibody

Cyclin G Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29306-1-AP

Synonyms

CCNG, CCNG1, CYCG1, Cyclin G, cyclin G1

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Validation Data Gallery

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  WB analysis using 29306-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29306-1-AP (Cyclin G antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: MDA-MB-231 cells, MDA-MB-453s cells, T-47D cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29306-1-AP targets Cyclin G in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cyclin G fusion protein Ag30937
• Full Name: cyclin G1
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC007093
• Gene Symbol: Cyclin G
• Gene ID (NCBI): 900
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cyclin G is also named as CCNG, CYCG1 and CCNG1, and and belongs to the cyclin family. Cyclin G may play a role in growth regulation. Cyclin G is associated with G2/M phase arrest in response to DNA damage. And it may be an intermediate by which p53 mediates its role as an inhibitor of cellular proliferation, and levels of Cyclin G are increased after induction of p53 by DNA damage (PMID:12015958). Cyclin G is high levels in skeletal muscle, ovary, kidney and colon. Cyclin G is very low levels in normal cells during G1 phase, which increase as cells enter the S phase and stay high throughout the S and G2/M phases. In breast cancer cells, consistent high levels of Cyclin G are found throughout the cell cycle (PMID:11063931).