Cyclin E1 Recombinant antibody, PBS Only (Capture)

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  Sandwich ELISA standard curve of MP00361-2

  Sandwich ELISA standard curve of MP00361-2, Human Cyclin E1 Monoclonal Matched Antibody Pair - PBS only. 82700-6-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag2110. 82700-8-PBS was HRP conjugated as the detection antibody. Range: 0.313-40 ng/mL.

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  Sample test of MP00361-2

  The mean human Cyclin E1 concentration was determined to be 132.4 ng/mL in Hela cell extract based on a 3.0 mg/mL extract load.

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  Cytometric bead array standard curve of MP00361-2

  Cytometric bead array standard curve of MP00361-2, Cyclin E1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82700-6-PBS. Detection antibody: 82700-8-PBS. Standard: Ag2110. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 82700-6-PBS

  Biolayer interferometry (BLl) kinetic assay of 82700-6-PBS against Human Cyclin E1 was performed. The affinity constant is 0.544 nM.

Cyclin E1 Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 82700-6-PBS

Clone No.240136C10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

CCNE1, G1/S-specific cyclin-E1, G1/S specific cyclin E1, Cyclin E, CCNE

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Product Information

82700-6-PBS targets Cyclin E1 as part of a matched antibody pair:

MP00361-2: 82700-6-PBS capture and 82700-8-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Cyclin E1 fusion protein Ag2110
• Full Name: cyclin E1
• Calculated Molecular Weight: 410 aa, 47 kDa
• GenBank Accession Number: BC035498
• Gene Symbol: Cyclin E1
• Gene ID (NCBI): 898
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P24864
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.