Cyclin A2 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

Cyclin A2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C6C11

Cat no : 66391-1-PBS

Synonyms

CCN1, CCNA, CCNA2, Cyclin A, Cyclin A2, cyclinA2

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Validation Data Gallery

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  WB analysis using 66391-1-Ig (same clone as 66391-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66391-1-Ig (Cyclin A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66391-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66391-1-Ig (same clone as 66391-1-PBS)

  WB result of Cyclin A2 antibody (66391-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclin A2 transfected HeLa cells. This data was developed using the same antibody clone with 66391-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66391-1-Ig (same clone as 66391-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66391-1-Ig (Cyclin A2 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66391-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66391-1-Ig (same clone as 66391-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66391-1-Ig (Cyclin A2 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66391-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66391-1-Ig (same clone as 66391-1-PBS)

  Immunofluorescent analysis of (4% PFA ) fixed HeLa cells using 66391-1-Ig(Cyclin A2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66391-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 66391-1-Ig (same clone as 66391-1-PBS)

  Immunofluorescent analysis of (4% PFA ) fixed MCF-7 cells using 66391-1-Ig(Cyclin A2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66391-1-PBS in a different storage buffer formulation.

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Product Information

66391-1-PBS targets Cyclin A2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Cyclin A2 fusion protein Ag12765
• Full Name: cyclin A2
• Calculated Molecular Weight: 432 aa, 49 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC104783
• Gene Symbol: Cyclin A2
• Gene ID (NCBI): 890
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CCNA2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. CCNA2 binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions.