Cyclin A2 Polyclonal antibody

• Featured Product
• KD/KO Validated

Cyclin A2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 27242-1-AP

Synonyms

CCN1, CCNA, CCNA2, Cyclin A, Cyclin A2, cyclinA2

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Validation Data Gallery

• 

  WB analysis using 27242-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27242-1-AP (Cyclin A2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 27242-1-AP

  WB result of Cyclin A2 antibody (27242-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclin A2 transfected HeLa cells.

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  WB analysis of U2OS using 27242-1-AP

  U2OS cells were subjected to SDS PAGE followed by western blot with 27242-1-AP (Cyclin A2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, U2OS cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below

Product Information

27242-1-AP targets Cyclin A2 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cyclin A2 fusion protein Ag25666
• Full Name: cyclin A2
• Calculated Molecular Weight: 432 aa, 49 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC104783
• Gene Symbol: Cyclin A2
• Gene ID (NCBI): 890
• RRID: AB_2880815
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cancer Sci; Methylation of NRN1 is a novel synthetic lethal marker of PI3K-Akt-mTOR and ATR inhibitors in esophageal cancer.; Authors - Wushuang Du
human	WB	Int J Mol Med; Morusin inhibits the growth of human colorectal cancer HCT116‑derived sphere‑forming cells via the inactivation of Akt pathway.; Authors - Yuqi Zhou
human	WB	Cancer Lett; PMID: 30872078; Authors - Jie Yang
human	WB	Biomed Pharmacother; Deapioplatycodin D promotes cell senescence induced by P21 through the mediation of incomplete mitophagy via BNIP3L; Authors - Yiquan Li
human	WB	Biochem Biophys Res Commun; Constructing and validating pan-apoptosis-related features for predicting prognosis and immunotherapy response in hepatocellular carcinoma; Authors - Yuhong He