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Cyclin A1 Polyclonal antibody, PBS Only

Cyclin A1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 13295-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cyclin A1 Polyclonal antibody (13295-1-AP)

    WB analysis using 13295-1-AP (same clone as 13295-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13295-1-AP (Cyclin A1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13295-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using Cyclin A1 Polyclonal antibody (13295-1-AP)

    IHC staining of mouse testis using 13295-1-AP (same clone as 13295-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13295-1-AP (Cyclin A1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13295-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using Cyclin A1 Polyclonal antibody (13295-1-AP)

    IHC staining of mouse testis using 13295-1-AP (same clone as 13295-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13295-1-AP (Cyclin A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13295-1-PBS in a different storage buffer formulation.

Product Information

13295-1-PBS targets Cyclin A1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cyclin A1 fusion protein Ag4005
Full Name cyclin A1
Calculated Molecular Weight 464 aa, 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC036346
Gene Symbol Cyclin A1
Gene ID (NCBI) 8900
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CCNA1 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. CCNA1 binds both CDK2 and CDC2 kinases, which give two distinct kinase activities, one appearing in S phase, the other in G2, and thus regulate separate functions in cell cycle. CCNA1 was found to bind to important cell cycle regulators, such as Rb family proteins, transcription factor E2F-1, and the p21 family proteins. In general, the expression of CCNA1 is tissue-specific and high CCNA1 expression is limited to testis; besides, lower levels of CCNA1 expression are also observed in other human cell lines and in healthy brain. Currently, CCNA1 expression has been illustrated to be downregulated in various tumors, including head and neck squamous-cell cancer (HNSCC) and nasopharyngeal carcinoma; and the promoter of the CCNA1 gene is found to be frequently methylated in colon cancer and HNSCC.