CXCL1 Polyclonal antibody

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  WB analysis using 30322-1-AP

  Untreated, LPS and Brefeldin A treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 30322-1-AP (Cxcl1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

CXCL1 Polyclonal Antibody for WB, ELISA

Cat No. 30322-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Synonyms

Growth-regulated alpha protein, chemokine (C X C motif) ligand 1

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Tested Applications

• Positive WB detected in: RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30322-1-AP targets CXCL1 in WB, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: fusion protein
• Full Name: chemokine (C-X-C motif) ligand 1
• Observed Molecular Weight: 11 kDa
• GenBank Accession Number: BC037997
• Gene Symbol: Cxcl1
• Gene ID (NCBI): 14825
• RRID: AB_3086293
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P12850
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CXCL1 a is a member of CXC family and also known as keratinocyte-derived chemokines (KC) or growth-related oncogene (GRO). CXCL1 is expressed by macrophages, neutrophils, and epithelial cells. CXCL1 binding specifically to the CXC chemokine receptor CXCR2, is involved in fibrogenesis and angiogenesis. This protein also plays a role in inflammation and as a chemoattractant for neutrophils.