Cullin 5 Polyclonal antibody, PBS Only

Cullin 5 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 31183-1-PBS

Synonyms

---

Validation Data Gallery

• 

  WB analysis using 31183-1-AP (same clone as 31183-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 31183-1-AP (Cullin 5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31183-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 31183-1-AP (same clone as 31183-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 31183-1-AP (Cullin 5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31183-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 31183-1-AP (same clone as 31183-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31183-1-AP (Cullin 5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31183-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 31183-1-AP (same clone as 31183-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31183-1-AP (Cullin 5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31183-1-PBS in a different storage buffer formulation.

---

Product Information

31183-1-PBS targets Cullin 5 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cullin 5 fusion protein Ag34620
• Full Name: cullin 5
• Calculated Molecular Weight: 91 kDa
• Observed Molecular Weight: 80 kDa, 90 kDa
• GenBank Accession Number: BC063306
• Gene Symbol: Cullin 5
• Gene ID (NCBI): 8065
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The cullin family proteins are scaffold proteins for the RING finger type E3 ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. In humans, there are eight members of cullins, including CUL1, 2, 3, 4A, 4B, 5, 7, and CUL9 with an evolutionarily conserved cullin homology domain at the C-terminus, responsible for their interaction with the RING protein, assembling 8 cullin-based RING dependent E3 ubiquitin ligases to target thousands of substrates for ubiquitination and degradation (PMID: 32334051). Cullin 5 is the key scaffolding protein of Cullin RING ligase-5 (CRL-5). Cullin 5 plays positive roles in regulating cell growth, proliferation and physiological and other processes in the human body (PMID: 32194910). Cullin 5 has been detected in the form of double bands in the results of Western blot, which might be caused by neddylation (PMID: 36449095; 31294695).