CstF-64 Monoclonal antibody

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  WB analysis using 68520-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68520-1-Ig (CstF-64 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

CstF-64 Monoclonal Antibody for WB, ELISA

Cat No. 68520-1-Ig

Clone No.1C5G2

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, ELISA

Synonyms

CF 1 64 kDa subunit, CstF 64, CSTF 64 kDa subunit, CSTF2

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Tested Applications

• Positive WB detected in: A549 cells, LNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68520-1-Ig targets CstF-64 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CstF-64 fusion protein Ag33751
• Full Name: cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa
• Calculated Molecular Weight: 61 kDa
• Observed Molecular Weight: 64-70 kDa
• GenBank Accession Number: BC017712
• Gene Symbol: CstF-64
• Gene ID (NCBI): 1478
• RRID: AB_3085229
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P33240
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cleavage stimulation factor 64 kDa subunit is a protein that in humans is encoded by the CSTF2 gene. This gene encodes a nuclear protein with an RRM (RNA recognition motif) domain. The protein is a member of the cleavage stimulation factor (CSTF) complex that is involved in the 3' end cleavage and polyadenylation of pre-mRNAs. Specifically, this protein binds GU-rich elements within the 3'-untranslated region of mRNAs.