• Featured Product
  • KD/KO Validated

CstF-64 Polyclonal antibody

CstF-64 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 26825-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

CF 1 64 kDa subunit, CstF 64, CSTF 64 kDa subunit, CSTF2, CstF-64

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration:  150 μL, 700 ug/ml
20 μL, 700 ug/ml
150 μL, 700 ug/ml

$449/ 150 μL

In stock in Rosemont, IL. Order within 40 hours and 47 mins to receive by Tuesday, April 8

Freight/Packing: $40.00

Quantity

Buy two full size antibodies and get a third full size primary antibody for free. Use promo code B2G125.


Proteintech Guarantee



Tested Applications

Positive WB detected inCOLO 320 cells, HeLa cells, mouse brain tissue, mouse testis tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26825-1-AP targets CstF-64 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CstF-64 fusion protein Ag25254 Predict reactive species
Full Name cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa
Calculated Molecular Weight 61 kDa
Observed Molecular Weight 64 kDa
GenBank Accession NumberBC017712
Gene Symbol CstF-64
Gene ID (NCBI) 1478
RRIDAB_2880649
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP33240
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cleavage stimulation factor 64 kDa subunit is a protein that in humans is encoded by the CSTF2 gene. This gene encodes a nuclear protein with an RRM (RNA recognition motif) domain. The protein is a member of the cleavage stimulation factor (CSTF) complex that is involved in the 3' end cleavage and polyadenylation of pre-mRNAs. Specifically, this protein binds GU-rich elements within the 3'-untranslated region of mRNAs.

Protocols

Product Specific Protocols
WB protocol for CstF-64 antibody 26825-1-APDownload protocol
IHC protocol for CstF-64 antibody 26825-1-APDownload protocol
IF protocol for CstF-64 antibody 26825-1-APDownload protocol
IP protocol for CstF-64 antibody 26825-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanCoIP

Neuron

Suppression of premature transcription termination leads to reduced mRNA isoform diversity and neurodegeneration.

Authors - Geneva R LaForce
humanIF

Biochem Biophys Res Commun

Cleavage stimulating factor 64 depletion mitigates cardiac fibrosis through alternative polyadenylation.

Authors - Rahul Neupane
  • KD Validated
mouseIHC

Front Oncol

CSTF2 Promotes Hepatocarcinogenesis and Hepatocellular Carcinoma Progression via Aerobic Glycolysis

Authors - Zhimin Chen
rat,mouse,human

iScience

Chromodomain helicase DNA-binding domain 2 maintains spermatogonial self-renewal by promoting chromatin accessibility and mRNA stability

Authors - Ziqian Min
humanWB

J Hepatocell Carcinoma

A Novel Gene Pair CSTF2/DPE2A Impacts Prognosis and Cell Cycle of Hepatocellular Carcinoma

Authors - Yangjin Huang
humanWB

Cell Discov

RBBP6 maintains glioblastoma stem cells through CPSF3-dependent alternative polyadenylation

Authors - Peng Lin
Loading...