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CoraLite647-conjugated F(ab')2 Fragment Goat Anti-Rabbit IgG (H+L)

Applications

IF, FC

Conjugate

CoraLite®647

Host

Goat

Reactivity

Rabbit

Cat no : SA00014-9

Validation Data Gallery

  • Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 10594-1-AP (PEX14 antiboody) and CoraLite®647 conjugated F(ab')2 Fragment Goat Anti-Rabbit IgG (H+L) antibody (SA00014-9) at dilution of 1:150.

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 10594-1-AP (PEX14 antiboody) and CoraLite®647 conjugated F(ab')2 Fragment Goat Anti-Rabbit IgG (H+L) antibody (SA00014-9) at dilution of 1:150.

  • 1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-human BCL2 Polyclonal antibody (12789-1-AP) and CoraLite®647-Conjugated F(ab')2 Fragment Goat Anti-Rabbit IgG (H+L) (SA00014-9) at dilution 1:1000 (red), and 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

    1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-human BCL2 Polyclonal antibody (12789-1-AP) and CoraLite®647-Conjugated F(ab')2 Fragment Goat Anti-Rabbit IgG (H+L) (SA00014-9) at dilution 1:1000 (red), and 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Product Information

Concentration 0.3 mg/mL
Applications IF, FC
Host Species Goat
Species Reactivity Rabbit
Physical State Liquid
Suggested Dilution Range 1:100 – 1:500
Purity The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Storage Buffer 0.01 M Sodium phosphate, 0.25 M NaCl, 50% glycerol, 5 mg/ml BSA, 0.01% Sodium Azide, pH 7.6.
Preservative0.01% Sodium Azide
Storage Instructions Store at -20°C. Stable for one year after shipment.
FluorophoreCL647 Fluorescent Dye. Amax=642nm, Emax=662nm
Stabilizer5 mg/ml BSA
Absorbance RatioA651/A280 = 4.2
ClonalityPolyclonal

Background

N/A

Publications

Title

Bone Res

Interorgan communication in neurogenic heterotopic ossification: the role of brain-derived extracellular vesicles

Authors - Weicheng Lu
View Article

Sci Rep

A novel role of peroxiredoxin 2 in diabetic kidney disease progression by activating the classically activated macrophages

Authors - Xia Li
View Article

J Exp Clin Cancer Res

TNFAIP2 confers cisplatin resistance in head and neck squamous cell carcinoma via KEAP1/NRF2 signaling

Authors - Teng Xu
View Article

J Control Release

A trinity STING-activating nanoparticle harnesses cancer cell STING machinery for enhanced immunotherapy

Authors - Yanming Xia
View Article

Sci Rep

Comparison of preparation methods of rat kidney single-cell suspensions

Authors - Tiantian Wang
View Article

Cell Prolif

Rack1-mediated ferroptosis affects hindgut development in rats with anorectal malformations: Spatial transcriptome insights

Authors - Chen-Yi Wang
View Article