CoraLite647-conjugated F(ab')2 Fragment Goat Anti-Mouse IgG (H+L)

Applications

IF, FC

Conjugate

CoraLite®647

Host

Goat

Reactivity

Mouse

Publications

14

Cat no : SA00014-10

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Validation Data Gallery

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  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66009-1-Ig (Beta Actin antiboody) and CoraLite®647 conjugated F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) antibody (SA00014-10) at dilution of 1:150.

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  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-human RBAP48 Monoclonal antibody (66060-1-Ig) and CoraLite®647-Conjugated F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-10) at dilution 1:1000 (red), and 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Product Information

• Concentration: 0.3 mg/mL
• Applications: IF, FC
• Host Species: Goat
• Species Reactivity: Mouse
• Physical State: Liquid
• Suggested Dilution Range: 1:100 – 1:500
• Purity: The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
• Storage Buffer: 0.01 M Sodium phosphate, 0.25 M NaCl, 50% glycerol, 5 mg/ml BSA, 0.01% Sodium Azide, pH 7.6.
• Preservative: 0.01% Sodium Azide
• Storage Instructions: Store at -20°C. Stable for one year after shipment.
• Fluorophore: CL647 Fluorescent Dye. Amax=642nm, Emax=662nm
• Stabilizer: 5 mg/ml BSA
• Absorbance Ratio: A651/A280 = 4.6
• Clonality: Polyclonal

Background

N/A

Publications

Title
Front Med; Distinct immune escape and microenvironment between RG-like and pri-OPC-like glioma revealed by single-cell RNA-seq analysis; Authors - Weiwei Xian
J Nanobiotechnology; GRP75-driven, cell-cycle-dependent macropinocytosis of Tat/pDNA-Ca2+ nanoparticles underlies distinct gene therapy effect in ovarian cancer.; Authors - Linjia Su
J Neuroinflammation; Conditional knockout of ASK1 in microglia/macrophages attenuates epileptic seizures and long-term neurobehavioural comorbidities by modulating the inflammatory responses of microglia/macrophages; Authors - Yiying Zhang
Mol Neurobiol; Surfeit Locus Protein 4 as a Novel Target for Therapeutic Intervention in Cerebral Ischemia-Reperfusion Injury; Authors - Wenjie Hu
Eur J Pharmacol; Cycloheptylprodigiosin from marine bacterium Spartinivicinus ruber MCCC 1K03745T induces a novel form of cell death characterized by Golgi disruption and enhanced secretion of cathepsin D in Non-small cell lung cancer cell lines; Authors - Xiaosi Lin
Sci Rep; A novel role of peroxiredoxin 2 in diabetic kidney disease progression by activating the classically activated macrophages; Authors - Xia Li

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