Product Information
Phalloidin is a toxin isolated from mushrooms that binds F-Actin in the cytoskeleton. This product is conjugated to our fluorescent CoraLite(R) dye to stain F-actin for labeling, identifying and quantifying actin filaments in immunostaining studies. Compared to many fluorescent dyes, CoraLite(R) dyes have advantages in brightness, light stability and water solubility. Excitation/Emission maxima wavelengths are 593 nm/614 nm
Conjugated
CoraLite®594-Phalloidin(red).
Ex/Em: 593/614 nm
Storage
Store at -20°C. Avoid exposure to light. Stable for 2 years after shipment.
Protocol
Stock preparation: Prepare a 200T/mL stock solution by dissolving lyophilized phalloidin (300T) in 1.5 mL sterile water and protect solution from light.
Cautions
This product is in the form of freeze-dried powder. Please centrifuge it immediately before use and dissolve it in a suitable solvent before use. Phalloidin is highly toxic, please take protective measures .
Stock preparation
Prepare a 200T/mL stock solution by dissolving lyophilized phalloidin (300T) in 1.5 mL sterile water and protect solution from light.
Cited in Article as
PF00003, CoraLite®594-Phalloidin (red), Proteintech, IL, USA
Publications
| Application | Title |
|---|---|
Imeta Hyperoside alleviates endometrial stromal cell senescence in unexplained recurrent spontaneous abortion via DHX9-mediated R-loop resolution. | |
Nat Commun ZNF274 constrains lineage plasticity and drives intrinsic resistance to CDK7 inhibitors in pancreatic cancer. | |
Nat Commun Mechanics-activated fibroblasts promote pulmonary group 2 innate lymphoid cell plasticity propelling silicosis progression | |
Metabolism Endothelial NPR-C promotes insulin resistance via Caveolin-1-mediated insulin transcytosis. | |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Angie (Verified Customer) (07-01-2024) | 4% PFA-fixed A549 cells were permeabilized with 0.1% Triton X-100 in PBS prior to staining. Cells were incubated with CoraLite®594-conjugated Phalloidin for 30 min at room temperature. The nuclei were counterstained with DAPI.
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FH Udesh (Verified Customer) (03-15-2024) | Significantly cheaper but equally good alternative to Life Tech's Phalloidin. Worked well at 1:400 in 2% BSA for 1 hour at room temperature
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FH Balawant (Verified Customer) (12-25-2023) | Very good antibody and with highly specific. This antibody has no background.
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