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CoraLite®488-Phalloidin (green)

Excitation Laser

490 nm

Emission

515 nm

Application

IF

Cat no : PF00001

Validation Data Gallery

  • Immunofluorescent analysis of (4% PFA) fixed A549 cells using the CoraLite®488-conjugated Phalloidin antibody, CL488-Phalloidin, at dilution of 1:400. The nuclei were counterstained with DAPI.

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using the CoraLite®488-conjugated Phalloidin antibody, CL488-Phalloidin, at dilution of 1:400. The nuclei were counterstained with DAPI.

  • NIH3T3 mouse fibroblasts. 1:50 dilution of CoraLite488-Phalloidin. Nuclei are stained with Hoechst 33342. Scale bar is 10 um. Images taken by Katarzyna Szymanksa-DeWijs from Hannover Medical School.

    NIH3T3 mouse fibroblasts. 1:50 dilution of CoraLite488-Phalloidin. Nuclei are stained with Hoechst 33342. Scale bar is 10 um. Images taken by Katarzyna Szymanksa-DeWijs from Hannover Medical School.

Product Information

Phalloidin is a toxin isolated from mushrooms that binds F-Actin in the cytoskeleton. This product is conjugated to our fluorescent CoraLite(R) dye to stain F-actin for labeling, identifying and quantifying actin filaments in immunostaining studies. Compared to many fluorescent dyes, CoraLite(R) dyes have advantages in brightness, light stability and water solubility. Excitation/Emission maxima wavelengths are 490 nm/515 nm.

Conjugated

CoraLite®488-Phalloidin (green)

Ex/Em: 490/515nm

Storage

Store at -20°C. Avoid exposure to light. Stable for 2 years after shipment. 

Cautions

This product is formulated as a freeze-dried powder. Please centrifuge it immediately before use and dissolve it in a suitable solvent before use. Phalloidin is highly toxic, please take protective measures while handling.

Cited in Article as

PF00001, CoraLite®488-Phalloidin (green), Proteintech, IL, USA

Publications

ApplicationTitle

Bioact Mater

A novel mechanism of inhibiting in-stent restenosis with arsenic trioxide drug-eluting stent: Enhancing contractile phenotype of vascular smooth muscle cells via YAP pathway.

Authors - Yinping Zhao
View Article
IF

Stem Cells Int

Erythropoietin Activates Autophagy to Regulate Apoptosis and Angiogenesis of Periodontal Ligament Stem Cells via the Akt/ERK1/2/BAD Signaling Pathway under Inflammatory Microenvironment

Authors - Denghao Huang
View Article
IF

Adv Healthc Mater

Photosensitive and Conductive Hydrogel Induced Innerved Bone Regeneration for Infected Bone Defect Repair

Authors - Xirui Jing
View Article
WB

Acta Biomater

Bioprinted living tissue constructs with layer-specific, growth factor-loaded microspheres for improved enthesis healing of a rotator cuff

Authors - Lang Bai
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Niklas (Verified Customer) (11-08-2022)

great staining with even a high dilution factor

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:400
  • Cell Tissue Type: human fibroblasts