CoraLite® Plus 488-Annexin V and PI Apoptosis Kit
Cat no : PF00005
Validation Data Gallery
Product Information
CoraLite® Plus 488-Annexin V and PI Apoptosis Detection Kit provides a quick and easy method to detect apoptosis by marking early apoptotic cells (green) and late apoptotic cells or necrotic cells (red).Results can be read out using flow cytometry or other fluorescence testing equipment. Early apoptic cells are identified by annexin V (labeled with CoraLite® Plus 488 dye) binding to phosphatidylserine (PS) flipped to the extracellular membrane surface, an early event in apoptosis. Compared with FITC, CoraLite® Plus 488 dye has higher fluorescence brightness, and is not affected by pH in the environment, and has good light stability.
Propidium lodide (PI) is a DNA-binding dye that can stain the nucleus of necrotic cells or cells that lose cell membrane integrity in the late stages of apoptosis. PI can be excited by lasers at 488, 532 or 546nm and CoraLite® Plus 488's excitation maximum is 490 nm.
| Components | 50 T | 100 T |
| 1×Annexin V Binding Buffer | 50 mL | 50 mL × 2 |
| CL488-Annexin V | 250 μL | 500 μL |
| PI | 500 μL | 1 mL |
Storage
Store at 4°C, Avoid exposure to light, DO NOT freeze. stable for 24 months after shipment.
Cautions
1. Please try to avoid light during storage.
2. For your safety and health, please wear lab coats and disposable gloves for operation.
3. For most accurate results, detection must performed as soon as possible after staining.
Spectral Characteristics
CL488-AnnexinV: Ex/Em=490/515nm
PI: Ex/Em=535/617nm (withDNA)
Cited in Article as
PF00005, CoraLite® Plus 488-Annexin V and PI Apoptosis Kit, Proteintech, IL, USA
Publications
| Application | Title |
|---|---|
ACS Appl Mater Interfaces Combined Chemo-Immuno-Photothermal Therapy for Effective Cancer Treatment via an All-in-One and One-for-All Nanoplatform | |
Molecules Naringenin Induces ROS-Mediated ER Stress, Autophagy, and Apoptosis in Human Osteosarcoma Cell Lines. | |
Pharmaceuticals (Basel) Identification of a Difluorinated Alkoxy Sulfonyl Chloride as a Novel Antitumor Agent for Hepatocellular Carcinoma through Activating Fumarate Hydratase Activity | |
J Ethnopharmacol Yiqi Jiedu decoction attenuates radiation injury of spermatogenic cells via suppressing IκBα/NF-κB pathway-induced excessive autophagy and apoptosis | |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Molly (Verified Customer) (01-10-2023) | Followed the example protocol and the kit worked really well.
|