CoraLite® Plus 488-Annexin V and PI Apoptosis Kit

PI: Ex/Em

535/617 nm

CL488-Annexin V: Ex/Em

490/515 nm



Cat no : PF00005

Product Information

CoraLite® Plus 488-Annexin V and PI Apoptosis Detection Kit provides a quick and easy method to detect apoptosis by marking early apoptotic cells (green) and late apoptotic cells or necrotic cells (red).Results can be read out using flow cytometry or other fluorescence testing equipment. Early apoptic cells are identified by annexin V (labeled with CoraLite® Plus 488 dye) binding to phosphatidylserine (PS) flipped to the extracellular membrane surface, an early event in apoptosis. Compared with FITC, CoraLite® Plus 488 dye has higher fluorescence brightness, and is not affected by pH in the environment, and has good light stability. 

Propidium lodide (PI) is a DNA-binding dye that can stain the nucleus of necrotic cells or cells that lose cell membrane integrity in the late stages of apoptosis. PI can be excited by lasers at 488, 532 or 546nm and CoraLite® Plus 488's excitation maximum is 490 nm.


 Components            50 T            100 T            
 1×Annexin V Binding Buffer            50 mL            50 mL × 2            
 CL488-Annexin V            250 μL            500 μL            
 PI500 μL1 mL


Store at 4°C,  Avoid exposure to light, DO NOT freeze. stable for 24 months after shipment.


1. Please try to avoid light during storage.

2. For your safety and health, please wear lab coats and disposable gloves for operation.

3. For most accurate results, detection must performed as soon as possible after staining.

Spectral Characteristics

CL488-AnnexinV: Ex/Em=490/515nm

PI: Ex/Em=535/617nm (withDNA)

Cited in Article as

PF00005, CoraLite® Plus 488-Annexin V and PI Apoptosis Kit, Proteintech, IL, USA



ACS Appl Mater Interfaces

Combined Chemo-Immuno-Photothermal Therapy for Effective Cancer Treatment via an All-in-One and One-for-All Nanoplatform

Authors - Xianglong Yu

Int J Nanomedicine

Role of Circulating Exosomal miRNA-3976 in Early Diabetic Retinopathy

Authors - Songtao Yang


Naringenin Induces ROS-Mediated ER Stress, Autophagy, and Apoptosis in Human Osteosarcoma Cell Lines.

Authors - Chiang-Wen Lee

Pharmaceuticals (Basel)

Identification of a Difluorinated Alkoxy Sulfonyl Chloride as a Novel Antitumor Agent for Hepatocellular Carcinoma through Activating Fumarate Hydratase Activity

Authors - Jin Jin

Nucleic Acids Res

Ribosomal frameshifting at normal codon repeats recodes functional chimeric proteins in human

Authors - Guiping Ren

J Ethnopharmacol

Yiqi Jiedu decoction attenuates radiation injury of spermatogenic cells via suppressing IκBα/NF-κB pathway-induced excessive autophagy and apoptosis

Authors - Xiaomeng Zhang


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Molly (Verified Customer) (01-10-2023)

Followed the example protocol and the kit worked really well.

  • Applications: Flow Cytometry
  • Cell Tissue Type: TC-32