Product Information
CoraLite® Plus 488-Annexin V and PI Apoptosis Detection Kit provides a quick and easy method to detect apoptosis by marking early apoptotic cells (green) and late apoptotic cells or necrotic cells (red).Results can be read out using flow cytometry or other fluorescence testing equipment. Early apoptic cells are identified by annexin V (labeled with CoraLite® Plus 488 dye) binding to phosphatidylserine (PS) flipped to the extracellular membrane surface, an early event in apoptosis. Compared with FITC, CoraLite® Plus 488 dye has higher fluorescence brightness, and is not affected by pH in the environment, and has good light stability.
Propidium lodide (PI) is a DNA-binding dye that can stain the nucleus of necrotic cells or cells that lose cell membrane integrity in the late stages of apoptosis. PI can be excited by lasers at 488, 532 or 546nm and CoraLite® Plus 488's excitation maximum is 490 nm.
| Components | 50 T | 100 T |
| 1×Annexin V Binding Buffer | 50 mL | 50 mL × 2 |
| CL488-Annexin V | 250 μL | 500 μL |
| PI | 500 μL | 1 mL |
Storage
Store at 4°C, Avoid exposure to light, DO NOT freeze. stable for 24 months after shipment.
Cautions
1. Please try to avoid light during storage.
2. For your safety and health, please wear lab coats and disposable gloves for operation.
3. For most accurate results, detection must performed as soon as possible after staining.
Spectral Characteristics
CL488-AnnexinV: Ex/Em=490/515nm
PI: Ex/Em=535/617nm (withDNA)
Cited in Article as
PF00005, CoraLite® Plus 488-Annexin V and PI Apoptosis Kit, Proteintech, IL, USA
Publications
| Application | Title |
|---|---|
Nucleic Acids Res Ribosomal frameshifting at normal codon repeats recodes functional chimeric proteins in human | |
J Exp Clin Cancer Res STRIP2 motivates non-small cell lung cancer progression by modulating the TMBIM6 stability through IGF2BP3 dependent | |
Sci Adv Repair of female reproductive function by GDF-9-overexpressing extracellular vesicles via ACVR1B/SMAD2 regulation in ovarian granulosa. | |
Med Large-scale quantitative metabolomics and mass spectrometry imaging define the metabolic atlas in human acute type A aortic dissection. | |
J Control Release In situ formed chemo-immunotherapeutic hydrogel for suppression of postoperative glioma recurrence and intraoperative hemostasis | |
NPJ Precis Oncol A machine learning-driven framework integrating cell death and senescence signatures for multi-target drug design and immunotherapy optimization in ovarian cancer. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Molly (Verified Customer) (01-10-2023) | Followed the example protocol and the kit worked really well.
|
