Connexin-50 Polyclonal antibody

Connexin-50 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 23161-1-AP

Synonyms

CX50, GJA8, GJA 8, Connexin50, Connexin 50

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Validation Data Gallery

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  WB analysis using 23161-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23161-1-AP (Connexin-50 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse eye tissue, rat eye tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

23161-1-AP targets Connexin-50 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Connexin-50 fusion protein Ag19572
• Full Name: gap junction protein, alpha 8, 50kDa
• Calculated Molecular Weight: 433 aa, 48 kDa
• Observed Molecular Weight: 60-65 kDa
• GenBank Accession Number: BC146507
• Gene Symbol: GJA8
• Gene ID (NCBI): 2703
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Connexin-50 (Cx50), also known as Gap junction alpha 8 protein (GJA8), belongs to the connexin family. Connexins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells. Connexin-50 is expressed in lens fiber cells and is essential for the normal physiological function of the lens (PMID: 11916859). Together with Connexin-46, Connexin-50 forms functional half-gap junction channels in non-junctional membranes. The expected molecular weight of Connexin-50 is 50 kDa, and in some literature, Connexin-50 was detected in lens samples at ~65 kDa (PMID: 31195409; PMID: 29196765).

Publications

Species	Application	Title
human	IF	Eur J Hum Genet; Association of variants in GJA8 with familial acorea-microphthalmia-cataract syndrome; Authors - Shuqian Dong