Connexin 43 Polyclonal antibody

Connexin 43 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse , rat and More (1)





Cat no : 26980-1-AP


Connexin 43, CX43, DFNB38, Gap junction alpha 1 protein, GJA1, GJAL, ODDD

Tested Applications

Positive WB detected inmouse brain tissue, mouse testis tissue, mouse heart tissue, rat brain tissue, rat heart tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inrat heart tissue, mouse heart tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse heart tissue, human heart tissue

Recommended dilution

Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26980-1-AP targets Connexin 43 in WB, IP, IF, FC, IHC, CoIP, ELISA applications and shows reactivity with human, mouse , rat samples.

Tested Reactivity human, mouse , rat
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Connexin 43 fusion protein Ag25533
Full Name gap junction protein, alpha 1, 43kDa
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 43-47 kDa
GenBank Accession NumberBC026329
Gene Symbol GJA1
Gene ID (NCBI) 2697
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Connexin-43 (Cx43, also known as gap junction alpha-1), a member of connexin family, plays essential roles in gap junction communication that facilitates direct communication among adjacent cells (PMID: 12270943). Usually, six connexin proteins oligomerize into a hemi-channel or connexon during intercellular channel formation (PMID: 12270943). Mutations of Cx43 may cause a series of diseases such as oculodentodigital dysplasia (ODDD), syndactyly 3 (SDTY3), hypoplastic left heart syndrome 1 (HLHS1) and so on (PMID: 18161618, 1472936, 1147490).


Product Specific Protocols
WB protocol for Connexin 43 antibody 26980-1-APDownload protocol
IHC protocol for Connexin 43 antibody 26980-1-APDownload protocol
IF protocol for Connexin 43 antibody 26980-1-APDownload protocol
IP protocol for Connexin 43 antibody 26980-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Muhammad (Verified Customer) (08-30-2022)

Cx43 antibody used is excellent and working in HUVEC cells.

  • Applications: Western Blot
  • Cell Tissue Type: HUVEC

ABDULLAH (Verified Customer) (11-08-2019)

This antibody worked great!It detected the protein at the right size.There wasn't any background.

  • Applications: Western Blot, Immunofluorescence,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: BEND.3 cells