Connexin-26 Polyclonal antibody

• Featured Product
• KD/KO Validated

Connexin-26 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA, IF

Conjugate

Unconjugated

Publications

1

Cat no : 14842-1-AP

Synonyms

Connexin 26, Connexin-26, CX26, DFNA3, DFNA3A, DFNB1, DFNB1A, Gap junction beta 2 protein, GJB2, HID, KID, NSRD1, PPK

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Validation Data Gallery

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  WB analysis of human heart using 14842-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14842-1-AP (Connexin-26 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 14842-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14842-1-AP (Connexin-26 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: human heart tissue, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Product Information

14842-1-AP targets Connexin-26 in WB, ELISA, IF applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: gap junction protein, beta 2, 26kDa
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: NM_004004
• Gene Symbol: Connexin-26
• Gene ID (NCBI): 2706
• RRID: AB_1607614
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The gap junctions were first characterized by electron microscopy as regionally specialized structures on plasma membranes of contacting adherent cells. These structures were shown to consist of cell-to-cell channels that facilitate the transfer of ions and small molecules between cells. The gap junction proteins, also known as connexins, purified from fractions of enriched gap junctions from different tissues differ. According to sequence similarities at the nucleotide and amino acid levels, the gap junction proteins are divided into two categories, alpha and beta. Connexin-26 (GJB2) is a beta class gap junction protein. Mutations in the gene for connexin-26 are responsible for as much as 50% of pre-lingual, recessive deafness.

Publications

Species	Application	Title
human	WB,IF	Elife; Solute exchange through gap junctions lessens the adverse effects of inactivating mutations in metabolite-handling genes; Authors - Stefania Monterisi ; KO Validated