Connexin-26 Polyclonal antibody

Connexin-26 Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 14842-1-AP

Synonyms

Connexin 26, Connexin-26, CX26, DFNA3, DFNA3A, DFNB1, DFNB1A, Gap junction beta 2 protein, GJB2, HID, KID, NSRD1, PPK



Tested Applications

Positive WB detected inhuman heart tissue, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14842-1-AP targets Connexin-26 in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name gap junction protein, beta 2, 26kDa
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberNM_004004
Gene Symbol GJB2
Gene ID (NCBI) 2706
RRIDAB_1607614
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The gap junctions were first characterized by electron microscopy as regionally specialized structures on plasma membranes of contacting adherent cells. These structures were shown to consist of cell-to-cell channels that facilitate the transfer of ions and small molecules between cells. The gap junction proteins, also known as connexins, purified from fractions of enriched gap junctions from different tissues differ. According to sequence similarities at the nucleotide and amino acid levels, the gap junction proteins are divided into two categories, alpha and beta. Connexin-26 (GJB2) is a beta class gap junction protein. Mutations in the gene for connexin-26 are responsible for as much as 50% of pre-lingual, recessive deafness.

Protocols

Product Specific Protocols
WB protocol for Connexin-26 antibody 14842-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Elife

Solute exchange through gap junctions lessens the adverse effects of inactivating mutations in metabolite-handling genes

Authors - Stefania Monterisi
  • KO Validated