Complement factor H antibody (12748-1-PBS)

Filter: AllWBIPIHCIF-PIF/ICC

WB analysis using 12748-1-AP (same clone as 12748-1-PBS)
human plasma were subjected to SDS PAGE followed by western blot with 12748-1-AP (Complement factor H antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12748-1-PBS in a different storage buffer formulation.
WB analysis of human blood using 12748-1-AP (same clone as 12748-1-PBS)
human blood were subjected to SDS PAGE followed by western blot with 12748-1-AP (Complement factor H antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12748-1-PBS in a different storage buffer formulation.
IP experiment of human plasma using 12748-1-AP (same clone as 12748-1-PBS)
IP result of anti-Complement factor H (IP:12748-1-AP, 4ug; Detection:12748-1-AP 1:1000) with human plasma lysate 4000ug. This data was developed using the same antibody clone with 12748-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 12748-1-AP (same clone as 12748-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12748-1-AP (Complement factor H antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12748-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 12748-1-AP (same clone as 12748-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12748-1-AP (Complement factor H antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12748-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 12748-1-AP (same clone as 12748-1-PBS)
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 12748-1-AP (Complement factor H antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12748-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 12748-1-AP (same clone as 12748-1-PBS)
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 12748-1-AP (Complement factor H antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12748-1-PBS in a different storage buffer formulation.
IF Staining of human liver cancer using 12748-1-AP (same clone as 12748-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Complement factor H antibody (12748-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12748-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 12748-1-AP (same clone as 12748-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Complement factor H antibody (12748-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 12748-1-PBS in a different storage buffer formulation.

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Tested Applications
Product Information

Product Information

12748-1-PBS targets Complement factor H in WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Complement factor H fusion protein Ag3482 Predict reactive species
Full Name	complement factor H
Calculated Molecular Weight	1231 aa, 139 kDa
Observed Molecular Weight	140-150 kDa
GenBank Accession Number	BC037285
Gene Symbol	Complement factor H
Gene ID (NCBI)	3075
RRID	AB_10642946
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P08603
Storage Buffer	PBS only , pH 7.3
Storage Conditions	Store at -80°C.

Background Information

Complement factor H (CFH) is an abundant plasma glycoprotein that regulates the function of the alternative complement pathway in the fluid phase and on cellular surfaces (PMID: 2963625). Factor H binds to C3b, accelerates the decay of the alternative pathway C3-convertase (C3bBb), and acts as a cofactor for the factor I-mediated proteolytic inactivation of C3b (PMID: 15163532). It plays a critical role in the homeostasis of the complement system in plasma and in the protection of bystander host cells and tissues from damage by complement activation. Abnormalities in factor H have been associated with renal disease (PMID: 18190458).