Complement factor D Recombinant antibody, PBS Only (Detector)

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  WB analysis using 84851-4-RR (same clone as 84851-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84851-4-RR (Complement factor D antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84851-4-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01660-3

  Sandwich ELISA standard curve of MP01660-3, Human Complement factor D Recombinant Matched Antibody Pair - PBS only. 84851-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1189. 84851-4-PBS was HRP conjugated as the detection antibody. Range: 4.69-300 pg/mL.

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  Sample test of MP01660-3

  Serum of eight individual healthy human donors was measured. The Complement factor D concentration of detected samples was determined to be 838.5 ng/mL with a range of 561.9-1525.4 ng/mL.

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  IHC staining of mouse stomach using 84851-4-RR (same clone as 84851-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 84851-4-RR (Complement factor D antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84851-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse stomach using 84851-4-RR (same clone as 84851-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 84851-4-RR (Complement factor D antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84851-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84851-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84851-4-RR against Human Complement factor D were performed. The affinity constant is 60.2 pM.

Complement factor D {ptg:Brand} Recombinant Antibody for WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 84851-4-PBS

Clone No.242244C2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

CFD, DF, complement factor D (adipsin), C3 convertase activator, ADIPSIN

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Product Information

84851-4-PBS targets Complement factor D as part of a matched antibody pair:

MP01660-3: 84851-2-PBS capture and 84851-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: complement factor D (adipsin)
• Calculated Molecular Weight: 27kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: NM_001928.3
• Gene Symbol: CFD
• Gene ID (NCBI): 1675
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P00746
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

Complement factor D, also known as adipsin, is a highly specific serine protease. Complement factor D is the rate-limiting enzyme in the alternative pathway of complement. It activates C3 convertase by cleaving factor B, which is a key step in the alternative pathway. Complement factor D catalyzes the cleavage of factor B to generate Ba (non-catalytic chain) and Bb (active catalytic subunit), which together with C3(H2O) (or C3b) constitute the active C3 convertase C3(H2O)Bb (or C3bBb). (PMID: 34566967; PMID: 27775713).