Collagen Type VI Monoclonal antibody, PBS Only

Collagen Type VI Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3E9C2

Cat no : 68652-1-PBS

Synonyms

COL6A2, Collagen alpha 2(VI) chain, collagen, type VI, alpha 2, DKFZp586E1322, FLJ46862, PP3610

---

Validation Data Gallery

• 

  WB analysis of human placenta using 68652-1-Ig (same clone as 68652-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 68652-1-Ig (COL6A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68652-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human heart using 68652-1-Ig (same clone as 68652-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 68652-1-Ig (COL6A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68652-1-PBS in a different storage buffer formulation.

---

Product Information

68652-1-PBS targets Collagen Type VI in WB, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Collagen Type VI fusion protein Ag7134
• Full Name: collagen, type VI, alpha 2
• Calculated Molecular Weight: 109 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: BC065509
• Gene Symbol: COL6A2
• Gene ID (NCBI): 1292
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Collagen VI, composed of three polypeptides, alpha-1(VI), alpha-2(VI), and alpha-3(VI) chains, is a beaded filament collagen found in most connective tissues. The alpha-1(VI) and alpha-2(VI) chains are 140 kDa, while the alpha-3(VI) chain is 200-300 kDa (PMID: 3792302, 3348212). COL6A2 gene encodes the alpha-2 subunit of type VI collagen. The alpha-2(VI) subunit has a calculated molecular weight of 109 kDa, and an apparent molecular weight of 140 kDa due to posttranslational modification. Mutations in COL6A2 gene are associated with Bethlem myopathy and Ullrich scleroatonic muscular dystrophy. Three transcript variants have been identified for this gene.