Collagen Type VI Polyclonal antibody

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  WB analysis using 31280-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31280-1-AP (COL6A3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Collagen Type VI Polyclonal Antibody for WB, ELISA

Cat No. 31280-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

COL6A3, Collagen alpha-3(VI) chain, Collagen alpha 3(VI) chain

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Tested Applications

• Positive WB detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31280-1-AP targets Collagen Type VI in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Collagen Type VI fusion protein Ag35065
• Full Name: collagen, type VI, alpha 3
• Calculated Molecular Weight: 344 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: NM_004369
• Gene Symbol: COL6A3
• Gene ID (NCBI): 1293
• RRID: AB_3669927
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P12111
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COL6A3 belongs to the type VI collagen family. Collagen VI acts as a cell-binding protein. It is expected to be expressed in smooth muscle. And the calculated molecular weight of COL6A3 is 343 kDa. The protein has similar expression in obese and T2DM (2 patients, and it regulates the chemotaxis and inflammation of macrophages in adipose tissue. Its expression is also related to weight gain. The expression of this protein in adipocytes is related to insulin resistance, which is believed to depend on PPAR (peroxisome proliferator-activated receptor) α-mediated adipocyte development (PMID: 25337653). Defects in COL6A3 are a cause of Bethlem myopathy (BM). Defects in COL6A3 are a cause of Ullrich congenital muscular dystrophy (UCMD) which also known as Ullrich scleroatonic muscular dystrophy. This antibody is specific to COL6A3.